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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthomonas campestris pv. campestris Queuine tRNA-ribosyltransferase (tgt) CSB-YP847907XAY
CSB-EP847907XAY
CSB-BP847907XAY
CSB-MP847907XAY
CSB-EP847907XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris DNA mismatch repair protein mutL (mutL), partial CSB-YP847908XAY
CSB-EP847908XAY
CSB-BP847908XAY
CSB-MP847908XAY
CSB-EP847908XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris UPF0061 protein XCC2284 (XCC2284), partial CSB-YP847909XAY
CSB-EP847909XAY
CSB-BP847909XAY
CSB-MP847909XAY
CSB-EP847909XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Cytochrome c-type biogenesis protein CcmE 2 (ccmE2), partial CSB-YP847910XAY
CSB-EP847910XAY
CSB-BP847910XAY
CSB-MP847910XAY
CSB-EP847910XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP847911XAY
CSB-EP847911XAY
CSB-BP847911XAY
CSB-MP847911XAY
CSB-EP847911XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP847912XAY
CSB-EP847912XAY
CSB-BP847912XAY
CSB-MP847912XAY
CSB-EP847912XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Outer-membrane lipoprotein carrier protein (lolA) CSB-YP847913XAY
CSB-EP847913XAY
CSB-BP847913XAY
CSB-MP847913XAY
CSB-EP847913XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Flagellar L-ring protein (flgH) CSB-YP847914XAY
CSB-EP847914XAY
CSB-BP847914XAY
CSB-MP847914XAY
CSB-EP847914XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP847915XAY
CSB-EP847915XAY
CSB-BP847915XAY
CSB-MP847915XAY
CSB-EP847915XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP847916XAY
CSB-EP847916XAY
CSB-BP847916XAY
CSB-MP847916XAY
CSB-EP847916XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Pantothenate synthetase (panC) CSB-YP847917XAY
CSB-EP847917XAY
CSB-BP847917XAY
CSB-MP847917XAY
CSB-EP847917XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Carbon storage regulator homolog (csrA) CSB-YP847918XAY
CSB-EP847918XAY
CSB-BP847918XAY
CSB-MP847918XAY
CSB-EP847918XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP847919XAY
CSB-EP847919XAY
CSB-BP847919XAY
CSB-MP847919XAY
CSB-EP847919XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP847920XAY1
CSB-EP847920XAY1
CSB-BP847920XAY1
CSB-MP847920XAY1
CSB-EP847920XAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L9 (rplI) CSB-YP847921XAY
CSB-EP847921XAY
CSB-BP847921XAY
CSB-MP847921XAY
CSB-EP847921XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Kynurenine 3-monooxygenase (kmo) CSB-YP847922XAY
CSB-EP847922XAY
CSB-BP847922XAY
CSB-MP847922XAY
CSB-EP847922XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Undecaprenyl pyrophosphate synthase (uppS) CSB-YP847923XAY
CSB-EP847923XAY
CSB-BP847923XAY
CSB-MP847923XAY
CSB-EP847923XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP847924XAY
CSB-EP847924XAY
CSB-BP847924XAY
CSB-MP847924XAY
CSB-EP847924XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD (rlmD) CSB-YP847925XAY
CSB-EP847925XAY
CSB-BP847925XAY
CSB-MP847925XAY
CSB-EP847925XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Catalase-peroxidase (katG), partial CSB-YP847926XAY
CSB-EP847926XAY
CSB-BP847926XAY
CSB-MP847926XAY
CSB-EP847926XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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