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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium meliloti Ribonuclease HII (rnhB) CSB-YP849950RKU
CSB-EP849950RKU
CSB-BP849950RKU
CSB-MP849950RKU
CSB-EP849950RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Histidine--tRNA ligase (hisS), partial CSB-YP849951RKU
CSB-EP849951RKU
CSB-BP849951RKU
CSB-MP849951RKU
CSB-EP849951RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti DNA gyrase inhibitor YacG (yacG) CSB-YP849952RKU
CSB-EP849952RKU
CSB-BP849952RKU
CSB-MP849952RKU
CSB-EP849952RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP849953RKU
CSB-EP849953RKU
CSB-BP849953RKU
CSB-MP849953RKU
CSB-EP849953RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 50S ribosomal protein L34 (rpmH) CSB-YP849954RKU
CSB-EP849954RKU
CSB-BP849954RKU
CSB-MP849954RKU
CSB-EP849954RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Methionyl-tRNA formyltransferase (fmt) CSB-YP849955RKU
CSB-EP849955RKU
CSB-BP849955RKU
CSB-MP849955RKU
CSB-EP849955RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Protein grpE (grpE) CSB-YP849956RKU
CSB-EP849956RKU
CSB-BP849956RKU
CSB-MP849956RKU
CSB-EP849956RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP849957RKU
CSB-EP849957RKU
CSB-BP849957RKU
CSB-MP849957RKU
CSB-EP849957RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Translation initiation factor IF-3 (infC) CSB-YP849958RKU
CSB-EP849958RKU
CSB-BP849958RKU
CSB-MP849958RKU
CSB-EP849958RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti tRNA pseudouridine synthase B (truB) CSB-YP849959RKU
CSB-EP849959RKU
CSB-BP849959RKU
CSB-MP849959RKU
CSB-EP849959RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Copper homeostasis protein CutC (cutC) CSB-YP849960RKU
CSB-EP849960RKU
CSB-BP849960RKU
CSB-MP849960RKU
CSB-EP849960RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP849961RKU
CSB-EP849961RKU
CSB-BP849961RKU
CSB-MP849961RKU
CSB-EP849961RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Tryptophan synthase beta chain (trpB) CSB-YP849962RKU
CSB-EP849962RKU
CSB-BP849962RKU
CSB-MP849962RKU
CSB-EP849962RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Acylphosphatase (acyP) CSB-YP849963RKU
CSB-EP849963RKU
CSB-BP849963RKU
CSB-MP849963RKU
CSB-EP849963RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Ureidoglycolate hydrolase 1 (allA1) CSB-YP849964RKU
CSB-EP849964RKU
CSB-BP849964RKU
CSB-MP849964RKU
CSB-EP849964RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti UPF0324 membrane protein RB0971 (RB0971), partial CSB-YP849965RKU
CSB-EP849965RKU
CSB-BP849965RKU
CSB-MP849965RKU
CSB-EP849965RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Urocanate hydratase (hutU), partial CSB-YP849966RKU
CSB-EP849966RKU
CSB-BP849966RKU
CSB-MP849966RKU
CSB-EP849966RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Arabinose import ATP-binding protein AraG (araG), partial CSB-YP849967RKU
CSB-EP849967RKU
CSB-BP849967RKU
CSB-MP849967RKU
CSB-EP849967RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Glutathione-dependent formaldehyde-activating enzyme (gfa) CSB-YP849968RKU
CSB-EP849968RKU
CSB-BP849968RKU
CSB-MP849968RKU
CSB-EP849968RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Opine oxidase subunit A (ooxA) CSB-YP849969RKU
CSB-EP849969RKU
CSB-BP849969RKU
CSB-MP849969RKU
CSB-EP849969RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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