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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria innocua serovar 6a Protoheme IX farnesyltransferase (ctaB), partial CSB-YP852929LGU
CSB-EP852929LGU
CSB-BP852929LGU
CSB-MP852929LGU
CSB-EP852929LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP852930LGU
CSB-EP852930LGU
CSB-BP852930LGU
CSB-MP852930LGU
CSB-EP852930LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP852931LGU
CSB-EP852931LGU
CSB-BP852931LGU
CSB-MP852931LGU
CSB-EP852931LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Ferredoxin--NADP reductase 1 (lin2075) CSB-YP852932LGU
CSB-EP852932LGU
CSB-BP852932LGU
CSB-MP852932LGU
CSB-EP852932LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Chorismate synthase (aroC) CSB-YP852933LGU
CSB-EP852933LGU
CSB-BP852933LGU
CSB-MP852933LGU
CSB-EP852933LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Pantothenate synthetase (panC) CSB-YP852934LGU
CSB-EP852934LGU
CSB-BP852934LGU
CSB-MP852934LGU
CSB-EP852934LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Transcriptional regulator MntR (mntR) CSB-YP852935LGU
CSB-EP852935LGU
CSB-BP852935LGU
CSB-MP852935LGU
CSB-EP852935LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP852936LGU
CSB-EP852936LGU
CSB-BP852936LGU
CSB-MP852936LGU
CSB-EP852936LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Dihydroorotase (pyrC) CSB-YP852937LGU
CSB-EP852937LGU
CSB-BP852937LGU
CSB-MP852937LGU
CSB-EP852937LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Guanylate kinase (gmk) CSB-YP852938LGU
CSB-EP852938LGU
CSB-BP852938LGU
CSB-MP852938LGU
CSB-EP852938LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Ribonuclease 3 (rnc) CSB-YP852939LGU
CSB-EP852939LGU
CSB-BP852939LGU
CSB-MP852939LGU
CSB-EP852939LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP852940LGU
CSB-EP852940LGU
CSB-BP852940LGU
CSB-MP852940LGU
CSB-EP852940LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP852941LGU
CSB-EP852941LGU
CSB-BP852941LGU
CSB-MP852941LGU
CSB-EP852941LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a UPF0374 protein lin1795 (lin1795) CSB-YP852942LGU
CSB-EP852942LGU
CSB-BP852942LGU
CSB-MP852942LGU
CSB-EP852942LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a 30S ribosomal protein S2 (rpsB) CSB-YP852943LGU
CSB-EP852943LGU
CSB-BP852943LGU
CSB-MP852943LGU
CSB-EP852943LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP852944LGU
CSB-EP852944LGU
CSB-BP852944LGU
CSB-MP852944LGU
CSB-EP852944LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP852945LGU
CSB-EP852945LGU
CSB-BP852945LGU
CSB-MP852945LGU
CSB-EP852945LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a UPF0173 metal-dependent hydrolase lin1612 (lin1612) CSB-YP852946LGU
CSB-EP852946LGU
CSB-BP852946LGU
CSB-MP852946LGU
CSB-EP852946LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Threonine--tRNA ligase (thrS), partial CSB-YP852947LGU
CSB-EP852947LGU
CSB-BP852947LGU
CSB-MP852947LGU
CSB-EP852947LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a 50S ribosomal protein L21 (rplU) CSB-YP852948LGU
CSB-EP852948LGU
CSB-BP852948LGU
CSB-MP852948LGU
CSB-EP852948LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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