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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Cleavage and polyadenylation specificity factor subunit 3-I (CPSF73-I), partial CSB-YP861257DOA
CSB-EP861257DOA
CSB-BP861257DOA
CSB-MP861257DOA
CSB-EP861257DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF070 (ERF070) CSB-YP861258DOA
CSB-EP861258DOA
CSB-BP861258DOA
CSB-MP861258DOA
CSB-EP861258DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L6-3 (RPL6C) CSB-YP861259DOA
CSB-EP861259DOA
CSB-BP861259DOA
CSB-MP861259DOA
CSB-EP861259DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative two-component response regulator-like APRR6 (APRR6), partial CSB-YP861260DOA
CSB-EP861260DOA
CSB-BP861260DOA
CSB-MP861260DOA
CSB-EP861260DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g71460, chloroplastic (PCMP-A3), partial CSB-YP861261DOA
CSB-EP861261DOA
CSB-BP861261DOA
CSB-MP861261DOA
CSB-EP861261DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable histone chaperone ASF1A (ASF1A) CSB-YP861262DOA
CSB-EP861262DOA
CSB-BP861262DOA
CSB-MP861262DOA
CSB-EP861262DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pumilio homolog 7, chloroplastic (APUM7), partial CSB-YP861263DOA
CSB-EP861263DOA
CSB-BP861263DOA
CSB-MP861263DOA
CSB-EP861263DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At1g73610 (At1g73610) CSB-YP861264DOA
CSB-EP861264DOA
CSB-BP861264DOA
CSB-MP861264DOA
CSB-EP861264DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Microtubule-associated protein 70-1 (MAP70.1), partial CSB-YP861265DOA
CSB-EP861265DOA
CSB-BP861265DOA
CSB-MP861265DOA
CSB-EP861265DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Thioredoxin H9 (TRX9) CSB-YP861266DOA
CSB-EP861266DOA
CSB-BP861266DOA
CSB-MP861266DOA
CSB-EP861266DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana V-type proton ATPase subunit E2 (VHA-E2) CSB-YP861267DOA
CSB-EP861267DOA
CSB-BP861267DOA
CSB-MP861267DOA
CSB-EP861267DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g74600, chloroplastic (PCMP-E69), partial CSB-YP861268DOA
CSB-EP861268DOA
CSB-BP861268DOA
CSB-MP861268DOA
CSB-EP861268DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Fucosyltransferase 3 (FUT3), partial CSB-YP861269DOA
CSB-EP861269DOA
CSB-BP861269DOA
CSB-MP861269DOA
CSB-EP861269DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g68980, mitochondrial (At1g68980), partial CSB-YP861270DOA
CSB-EP861270DOA
CSB-BP861270DOA
CSB-MP861270DOA
CSB-EP861270DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 4 (CRRSP4) CSB-YP861271DOA
CSB-EP861271DOA
CSB-BP861271DOA
CSB-MP861271DOA
CSB-EP861271DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Lipoxygenase 6, choloroplastic (LOX6), partial CSB-YP861272DOA
CSB-EP861272DOA
CSB-BP861272DOA
CSB-MP861272DOA
CSB-EP861272DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein phosphatase 2C 16 (HAB1) CSB-YP861273DOA
CSB-EP861273DOA
CSB-BP861273DOA
CSB-MP861273DOA
CSB-EP861273DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g63070, mitochondrial (At1g63070), partial CSB-YP861274DOA
CSB-EP861274DOA
CSB-BP861274DOA
CSB-MP861274DOA
CSB-EP861274DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative peptide/nitrate transporter At1g69860 (At1g69860), partial CSB-YP861275DOA
CSB-EP861275DOA
CSB-BP861275DOA
CSB-MP861275DOA
CSB-EP861275DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine/threonine-protein kinase Nek2 (NEK2), partial CSB-YP861276DOA
CSB-EP861276DOA
CSB-BP861276DOA
CSB-MP861276DOA
CSB-EP861276DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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