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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus halodurans 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP862581BQS
CSB-EP862581BQS
CSB-BP862581BQS
CSB-MP862581BQS
CSB-EP862581BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0178 protein BH1374 (BH1374) CSB-YP862582BQS
CSB-EP862582BQS
CSB-BP862582BQS
CSB-MP862582BQS
CSB-EP862582BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Probable rRNA maturation factor (BH1363) CSB-YP862583BQS
CSB-EP862583BQS
CSB-BP862583BQS
CSB-MP862583BQS
CSB-EP862583BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Heat-inducible transcription repressor HrcA (hrcA) CSB-YP862584BQS
CSB-EP862584BQS
CSB-BP862584BQS
CSB-MP862584BQS
CSB-EP862584BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Transcription elongation factor GreA (greA) CSB-YP862585BQS
CSB-EP862585BQS
CSB-BP862585BQS
CSB-MP862585BQS
CSB-EP862585BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Histidine--tRNA ligase (hisS) CSB-YP862586BQS
CSB-EP862586BQS
CSB-BP862586BQS
CSB-MP862586BQS
CSB-EP862586BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Queuine tRNA-ribosyltransferase (tgt) CSB-YP862587BQS
CSB-EP862587BQS
CSB-BP862587BQS
CSB-MP862587BQS
CSB-EP862587BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0735 ACT domain-containing protein BH1214 (BH1214) CSB-YP862588BQS
CSB-EP862588BQS
CSB-BP862588BQS
CSB-MP862588BQS
CSB-EP862588BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 3'-5' exoribonuclease yhaM (yhaM) CSB-YP862589BQS
CSB-EP862589BQS
CSB-BP862589BQS
CSB-MP862589BQS
CSB-EP862589BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Glycogen synthase (glgA) CSB-YP862590BQS
CSB-EP862590BQS
CSB-BP862590BQS
CSB-MP862590BQS
CSB-EP862590BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Chaperone protein htpG (htpG), partial CSB-YP862591BQS
CSB-EP862591BQS
CSB-BP862591BQS
CSB-MP862591BQS
CSB-EP862591BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Regulatory protein recX (recX) CSB-YP862592BQS
CSB-EP862592BQS
CSB-BP862592BQS
CSB-MP862592BQS
CSB-EP862592BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans L-arabinose transport system permease protein AraP (araP), partial CSB-YP862593BQS1
CSB-EP862593BQS1
CSB-BP862593BQS1
CSB-MP862593BQS1
CSB-EP862593BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 5-hydroxyisourate hydrolase (BH0760) CSB-YP862594BQS
CSB-EP862594BQS
CSB-BP862594BQS
CSB-MP862594BQS
CSB-EP862594BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP862595BQS
CSB-EP862595BQS
CSB-BP862595BQS
CSB-MP862595BQS
CSB-EP862595BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP862596BQS
CSB-EP862596BQS
CSB-BP862596BQS
CSB-MP862596BQS
CSB-EP862596BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP862597BQS
CSB-EP862597BQS
CSB-BP862597BQS
CSB-MP862597BQS
CSB-EP862597BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Phosphonates import ATP-binding protein PhnC 2 (phnC2) CSB-YP862598BQS
CSB-EP862598BQS
CSB-BP862598BQS
CSB-MP862598BQS
CSB-EP862598BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 50S ribosomal protein L11-2 (rplK2) CSB-YP862599BQS
CSB-EP862599BQS
CSB-BP862599BQS
CSB-MP862599BQS
CSB-EP862599BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Uncharacterized protein BH0262 (BH0262) CSB-YP862600BQS
CSB-EP862600BQS
CSB-BP862600BQS
CSB-MP862600BQS
CSB-EP862600BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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