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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana CMP-sialic acid transporter 3 (UTR6), partial CSB-YP863205DOA
CSB-EP863205DOA
CSB-BP863205DOA
CSB-MP863205DOA
CSB-EP863205DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein TIFY 3B (TIFY3B) CSB-YP863206DOA
CSB-EP863206DOA
CSB-BP863206DOA
CSB-MP863206DOA
CSB-EP863206DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcineurin B-like protein 6 (CBL6) CSB-YP863207DOA
CSB-EP863207DOA
CSB-BP863207DOA
CSB-MP863207DOA
CSB-EP863207DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Classical arabinogalactan protein 9 (AGP9) CSB-YP863208DOA
CSB-EP863208DOA
CSB-BP863208DOA
CSB-MP863208DOA
CSB-EP863208DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histidine kinase 2 (AHK2), partial CSB-YP863209DOA
CSB-EP863209DOA
CSB-BP863209DOA
CSB-MP863209DOA
CSB-EP863209DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ATX1 (ATX1), partial CSB-YP863210DOA
CSB-EP863210DOA
CSB-BP863210DOA
CSB-MP863210DOA
CSB-EP863210DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative syntaxin-24 (SYP24) CSB-YP863211DOA
CSB-EP863211DOA
CSB-BP863211DOA
CSB-MP863211DOA
CSB-EP863211DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Elongation factor G, mitochondrial (At1g45332) CSB-YP863212DOA
CSB-EP863212DOA
CSB-BP863212DOA
CSB-MP863212DOA
CSB-EP863212DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable phosphatidylinositol 4-kinase type 2-beta At1g26270 (At1g26270), partial CSB-YP863213DOA
CSB-EP863213DOA
CSB-BP863213DOA
CSB-MP863213DOA
CSB-EP863213DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative protease Do-like 6, chloroplastic (DEGP6) CSB-YP863214DOA
CSB-EP863214DOA
CSB-BP863214DOA
CSB-MP863214DOA
CSB-EP863214DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphoethanolamine N-methyltransferase 3 (NMT3) CSB-YP863215DOA
CSB-EP863215DOA
CSB-BP863215DOA
CSB-MP863215DOA
CSB-EP863215DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative FBD-associated F-box protein At1g50980 (At1g50980) CSB-YP863216DOA
CSB-EP863216DOA
CSB-BP863216DOA
CSB-MP863216DOA
CSB-EP863216DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Adenylate isopentenyltransferase 6, chloroplastic (IPT6) CSB-YP863217DOA
CSB-EP863217DOA
CSB-BP863217DOA
CSB-MP863217DOA
CSB-EP863217DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UPF0725 protein At1g27860 (At1g27860) CSB-YP863218DOA
CSB-EP863218DOA
CSB-BP863218DOA
CSB-MP863218DOA
CSB-EP863218DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g31920 (PCMP-H11), partial CSB-YP863219DOA
CSB-EP863219DOA
CSB-BP863219DOA
CSB-MP863219DOA
CSB-EP863219DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Secretory carrier-associated membrane protein 4 (SCAMP4), partial CSB-YP863220DOA1
CSB-EP863220DOA1
CSB-BP863220DOA1
CSB-MP863220DOA1
CSB-EP863220DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At1g55070 (At1g55070) CSB-YP863221DOA
CSB-EP863221DOA
CSB-BP863221DOA
CSB-MP863221DOA
CSB-EP863221DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Telomere repeat-binding protein 3 (TRP3), partial CSB-YP863222DOA
CSB-EP863222DOA
CSB-BP863222DOA
CSB-MP863222DOA
CSB-EP863222DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor TCP22 (TCP22) CSB-YP863223DOA
CSB-EP863223DOA
CSB-BP863223DOA
CSB-MP863223DOA
CSB-EP863223DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative zinc finger CCCH domain-containing protein 10 (At1g29600/At1g29610) CSB-YP863224DOA
CSB-EP863224DOA
CSB-BP863224DOA
CSB-MP863224DOA
CSB-EP863224DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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