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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (EMB1467), partial CSB-YP863745DOA
CSB-EP863745DOA
CSB-BP863745DOA
CSB-MP863745DOA
CSB-EP863745DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Aquaporin TIP2-3 (TIP2-3), partial CSB-YP863746DOA1
CSB-EP863746DOA1
CSB-BP863746DOA1
CSB-MP863746DOA1
CSB-EP863746DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein DYAD (DYAD), partial CSB-YP863747DOA
CSB-EP863747DOA
CSB-BP863747DOA
CSB-MP863747DOA
CSB-EP863747DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cleavage and polyadenylation specificity factor subunit 1 (CPSF160), partial CSB-YP863748DOA
CSB-EP863748DOA
CSB-BP863748DOA
CSB-MP863748DOA
CSB-EP863748DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana C2 and GRAM domain-containing protein At5g50170 (At5g50170), partial CSB-YP863749DOA
CSB-EP863749DOA
CSB-BP863749DOA
CSB-MP863749DOA
CSB-EP863749DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g49610 (At5g49610) CSB-YP863750DOA
CSB-EP863750DOA
CSB-BP863750DOA
CSB-MP863750DOA
CSB-EP863750DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine carboxypeptidase-like 41 (SCPL41) CSB-YP863751DOA
CSB-EP863751DOA
CSB-BP863751DOA
CSB-MP863751DOA
CSB-EP863751DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable galacturonosyltransferase 12 (GAUT12), partial CSB-YP863752DOA
CSB-EP863752DOA
CSB-BP863752DOA
CSB-MP863752DOA
CSB-EP863752DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-amylase 8 (BAM8), partial CSB-YP863753DOA
CSB-EP863753DOA
CSB-BP863753DOA
CSB-MP863753DOA
CSB-EP863753DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutathione S-transferase T3 (GSTT3), partial CSB-YP863754DOA
CSB-EP863754DOA
CSB-BP863754DOA
CSB-MP863754DOA
CSB-EP863754DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable L-type lectin-domain containing receptor kinase S.7 (LECRKS7), partial CSB-YP863755DOA
CSB-EP863755DOA
CSB-BP863755DOA
CSB-MP863755DOA
CSB-EP863755DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At5g36200 (At5g36200) CSB-YP863756DOA
CSB-EP863756DOA
CSB-BP863756DOA
CSB-MP863756DOA
CSB-EP863756DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Kinesin-like calmodulin-binding protein (KCBP), partial CSB-YP863757DOA
CSB-EP863757DOA
CSB-BP863757DOA
CSB-MP863757DOA
CSB-EP863757DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At5g43190 (At5g43190) CSB-YP863758DOA
CSB-EP863758DOA
CSB-BP863758DOA
CSB-MP863758DOA
CSB-EP863758DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein ABIL4 (ABIL4) CSB-YP863759DOA
CSB-EP863759DOA
CSB-BP863759DOA
CSB-MP863759DOA
CSB-EP863759DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Defensin-like protein 17 (PDF1.2C) CSB-YP863760DOA
CSB-EP863760DOA
CSB-BP863760DOA
CSB-MP863760DOA
CSB-EP863760DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chaperone protein ClpC1, chloroplastic (CLPC1), partial CSB-YP863761DOA
CSB-EP863761DOA
CSB-BP863761DOA
CSB-MP863761DOA
CSB-EP863761DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable ferredoxin-4, chloroplastic (FD4) CSB-YP863762DOA
CSB-EP863762DOA
CSB-BP863762DOA
CSB-MP863762DOA
CSB-EP863762DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable receptor-like protein kinase At5g39030 (At5g39030), partial CSB-YP863763DOA
CSB-EP863763DOA
CSB-BP863763DOA
CSB-MP863763DOA
CSB-EP863763DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable L-type lectin-domain containing receptor kinase II.1 (LECRK21), partial CSB-YP863764DOA
CSB-EP863764DOA
CSB-BP863764DOA
CSB-MP863764DOA
CSB-EP863764DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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