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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Regulatory-associated protein of TOR 2 (RAPTOR2), partial CSB-YP864906DOA
CSB-EP864906DOA
CSB-BP864906DOA
CSB-MP864906DOA
CSB-EP864906DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Scarecrow-like protein 22 (SCL22), partial CSB-YP864907DOA
CSB-EP864907DOA
CSB-BP864907DOA
CSB-MP864907DOA
CSB-EP864907DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 76F2 (UGT76F2) CSB-YP864908DOA
CSB-EP864908DOA
CSB-BP864908DOA
CSB-MP864908DOA
CSB-EP864908DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF043 (ERF043) CSB-YP864909DOA
CSB-EP864909DOA
CSB-BP864909DOA
CSB-MP864909DOA
CSB-EP864909DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine carboxypeptidase 24 (SCPL24) CSB-YP864910DOA
CSB-EP864910DOA
CSB-BP864910DOA
CSB-MP864910DOA
CSB-EP864910DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/LRR-repeat protein At4g29420 (At4g29420) CSB-YP864911DOA
CSB-EP864911DOA
CSB-BP864911DOA
CSB-MP864911DOA
CSB-EP864911DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Leucine-rich repeat receptor-like protein kinase PXL2 (PXL2), partial CSB-YP864912DOA
CSB-EP864912DOA
CSB-BP864912DOA
CSB-MP864912DOA
CSB-EP864912DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At4g25040 (At4g25040), partial CSB-YP864913DOA1
CSB-EP864913DOA1
CSB-BP864913DOA1
CSB-MP864913DOA1
CSB-EP864913DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH81 (BHLH81) CSB-YP864914DOA
CSB-EP864914DOA
CSB-BP864914DOA
CSB-MP864914DOA
CSB-EP864914DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Isoamylase 3, chloroplastic (ISA3), partial CSB-YP864915DOA
CSB-EP864915DOA
CSB-BP864915DOA
CSB-MP864915DOA
CSB-EP864915DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein SKIP19 (SKIP19) CSB-YP864916DOA
CSB-EP864916DOA
CSB-BP864916DOA
CSB-MP864916DOA
CSB-EP864916DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana K (+) efflux antiporter 3, chloroplastic (KEA3), partial CSB-YP864917DOA
CSB-EP864917DOA
CSB-BP864917DOA
CSB-MP864917DOA
CSB-EP864917DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH57 (BHLH57) CSB-YP864918DOA
CSB-EP864918DOA
CSB-BP864918DOA
CSB-MP864918DOA
CSB-EP864918DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative nitrate excretion transporter 7 (At3g45720), partial CSB-YP864919DOA
CSB-EP864919DOA
CSB-BP864919DOA
CSB-MP864919DOA
CSB-EP864919DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable fructokinase-4 (At3g59480) CSB-YP864920DOA
CSB-EP864920DOA
CSB-BP864920DOA
CSB-MP864920DOA
CSB-EP864920DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable nitrate excretion transporter 2 (At3g45660), partial CSB-YP864921DOA
CSB-EP864921DOA
CSB-BP864921DOA
CSB-MP864921DOA
CSB-EP864921DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter F family member 4 (ABCF4), partial CSB-YP864922DOA
CSB-EP864922DOA
CSB-BP864922DOA
CSB-MP864922DOA
CSB-EP864922DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphatidylinositol 4-phosphate 5-kinase 4 (PIP5K4), partial CSB-YP864923DOA
CSB-EP864923DOA
CSB-BP864923DOA
CSB-MP864923DOA
CSB-EP864923DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable glutamate carboxypeptidase 2 (AMP1), partial CSB-YP864924DOA
CSB-EP864924DOA
CSB-BP864924DOA
CSB-MP864924DOA
CSB-EP864924DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ribosome-recycling factor, chloroplastic (RRF) CSB-YP864925DOA
CSB-EP864925DOA
CSB-BP864925DOA
CSB-MP864925DOA
CSB-EP864925DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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