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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Dihydroneopterin aldolase (FOLB1) CSB-YP865867DOA
CSB-EP865867DOA
CSB-BP865867DOA
CSB-MP865867DOA
CSB-EP865867DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g09650, chloroplastic (HCF152), partial CSB-YP865868DOA
CSB-EP865868DOA
CSB-BP865868DOA
CSB-MP865868DOA
CSB-EP865868DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At3g09930 (At3g09930) CSB-YP865869DOA
CSB-EP865869DOA
CSB-BP865869DOA
CSB-MP865869DOA
CSB-EP865869DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF012 (ERF012) CSB-YP865870DOA
CSB-EP865870DOA
CSB-BP865870DOA
CSB-MP865870DOA
CSB-EP865870DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Delta-aminolevulinic acid dehydratase, chloroplastic (HEMB) CSB-YP865871DOA
CSB-EP865871DOA
CSB-BP865871DOA
CSB-MP865871DOA
CSB-EP865871DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein NLP5 (NLP5), partial CSB-YP865872DOA
CSB-EP865872DOA
CSB-BP865872DOA
CSB-MP865872DOA
CSB-EP865872DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pectinesterase/pectinesterase inhibitor 24 (PME24), partial CSB-YP865873DOA1
CSB-EP865873DOA1
CSB-BP865873DOA1
CSB-MP865873DOA1
CSB-EP865873DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 83A1 (UGT83A1) CSB-YP865874DOA
CSB-EP865874DOA
CSB-BP865874DOA
CSB-MP865874DOA
CSB-EP865874DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Methyl-CpG-binding domain-containing protein 9 (MBD9), partial CSB-YP865875DOA
CSB-EP865875DOA
CSB-BP865875DOA
CSB-MP865875DOA
CSB-EP865875DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g76280 (At1g76280), partial CSB-YP865876DOA
CSB-EP865876DOA
CSB-BP865876DOA
CSB-MP865876DOA
CSB-EP865876DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative proline-rich receptor-like protein kinase PERK11 (PERK11), partial CSB-YP865877DOA
CSB-EP865877DOA
CSB-BP865877DOA
CSB-MP865877DOA
CSB-EP865877DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative flavin-containing monooxygenase FMO GS-OX-like 11 (At1g63390) CSB-YP865878DOA
CSB-EP865878DOA
CSB-BP865878DOA
CSB-MP865878DOA
CSB-EP865878DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Reticulon-like protein B3 (RTNLB3), partial CSB-YP865879DOA1
CSB-EP865879DOA1
CSB-BP865879DOA1
CSB-MP865879DOA1
CSB-EP865879DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger protein ZAT4 (ZAT4) CSB-YP865880DOA
CSB-EP865880DOA
CSB-BP865880DOA
CSB-MP865880DOA
CSB-EP865880DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein argonaute 3 (AGO3), partial CSB-YP865881DOA
CSB-EP865881DOA
CSB-BP865881DOA
CSB-MP865881DOA
CSB-EP865881DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Leucine-rich repeat receptor-like serine/threonine-protein kinase At1g17230 (At1g17230), partial CSB-YP865882DOA
CSB-EP865882DOA
CSB-BP865882DOA
CSB-MP865882DOA
CSB-EP865882DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g06580 (At1g06580) CSB-YP865883DOA
CSB-EP865883DOA
CSB-BP865883DOA
CSB-MP865883DOA
CSB-EP865883DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutamate receptor 2.2 (GLR2.2), partial CSB-YP865884DOA
CSB-EP865884DOA
CSB-BP865884DOA
CSB-MP865884DOA
CSB-EP865884DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pre-mRNA-splicing factor SLU7-A (At1g65660), partial CSB-YP865885DOA
CSB-EP865885DOA
CSB-BP865885DOA
CSB-MP865885DOA
CSB-EP865885DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Purple acid phosphatase 11 (PAP11) CSB-YP865886DOA
CSB-EP865886DOA
CSB-BP865886DOA
CSB-MP865886DOA
CSB-EP865886DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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