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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neisseria meningitidis serogroup A / serotype 4A RNA pyrophosphohydrolase (rppH) CSB-YP867579NGF
CSB-EP867579NGF
CSB-BP867579NGF
CSB-MP867579NGF
CSB-EP867579NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A UPF0237 protein NMA1909 (NMA1909) CSB-YP867580NGF
CSB-EP867580NGF
CSB-BP867580NGF
CSB-MP867580NGF
CSB-EP867580NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Ribonuclease HI (rnhA) CSB-YP867581NGF
CSB-EP867581NGF
CSB-BP867581NGF
CSB-MP867581NGF
CSB-EP867581NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP867582NGF
CSB-EP867582NGF
CSB-BP867582NGF
CSB-MP867582NGF
CSB-EP867582NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Chaperone protein ClpB (clpB), partial CSB-YP867583NGF
CSB-EP867583NGF
CSB-BP867583NGF
CSB-MP867583NGF
CSB-EP867583NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Ribosome-binding factor A (rbfA) CSB-YP867584NGF
CSB-EP867584NGF
CSB-BP867584NGF
CSB-MP867584NGF
CSB-EP867584NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Proline--tRNA ligase (proS), partial CSB-YP867585NGF
CSB-EP867585NGF
CSB-BP867585NGF
CSB-MP867585NGF
CSB-EP867585NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A DNA translocase FtsK 1 (ftsK1), partial CSB-YP867586NGF
CSB-EP867586NGF
CSB-BP867586NGF
CSB-MP867586NGF
CSB-EP867586NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A 3-isopropylmalate dehydrogenase (leuB) CSB-YP867587NGF
CSB-EP867587NGF
CSB-BP867587NGF
CSB-MP867587NGF
CSB-EP867587NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP867588NGF
CSB-EP867588NGF
CSB-BP867588NGF
CSB-MP867588NGF
CSB-EP867588NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Chaperone protein hscA homolog (hscA), partial CSB-YP867589NGF
CSB-EP867589NGF
CSB-BP867589NGF
CSB-MP867589NGF
CSB-EP867589NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Protein CrcB homolog (crcB), partial CSB-YP867590NGF1
CSB-EP867590NGF1
CSB-BP867590NGF1
CSB-MP867590NGF1
CSB-EP867590NGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP867591NGF
CSB-EP867591NGF
CSB-BP867591NGF
CSB-MP867591NGF
CSB-EP867591NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Proline iminopeptidase (pip) CSB-YP867592NGF
CSB-EP867592NGF
CSB-BP867592NGF
CSB-MP867592NGF
CSB-EP867592NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Histidine--tRNA ligase (hisS) CSB-YP867593NGF
CSB-EP867593NGF
CSB-BP867593NGF
CSB-MP867593NGF
CSB-EP867593NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP867594NGF
CSB-EP867594NGF
CSB-BP867594NGF
CSB-MP867594NGF
CSB-EP867594NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP867595NGF
CSB-EP867595NGF
CSB-BP867595NGF
CSB-MP867595NGF
CSB-EP867595NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A 50S ribosomal protein L20 (rplT) CSB-YP867596NGF
CSB-EP867596NGF
CSB-BP867596NGF
CSB-MP867596NGF
CSB-EP867596NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP867597NGF
CSB-EP867597NGF
CSB-BP867597NGF
CSB-MP867597NGF
CSB-EP867597NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Bis (5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) CSB-YP867598NGF
CSB-EP867598NGF
CSB-BP867598NGF
CSB-MP867598NGF
CSB-EP867598NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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