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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Phosphatidylinositol 4-phosphate 5-kinase 6 (PIP5K6), partial CSB-YP871051DOA
CSB-EP871051DOA
CSB-BP871051DOA
CSB-MP871051DOA
CSB-EP871051DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Filament-like plant protein 2 (FPP2), partial CSB-YP871052DOA
CSB-EP871052DOA
CSB-BP871052DOA
CSB-MP871052DOA
CSB-EP871052DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L13a-1 (RPL13AA) CSB-YP871053DOA
CSB-EP871053DOA
CSB-BP871053DOA
CSB-MP871053DOA
CSB-EP871053DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cyclin-B2-4 (CYCB2-4) CSB-YP871054DOA
CSB-EP871054DOA
CSB-BP871054DOA
CSB-MP871054DOA
CSB-EP871054DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g10790 (At3g10790) CSB-YP871055DOA
CSB-EP871055DOA
CSB-BP871055DOA
CSB-MP871055DOA
CSB-EP871055DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone H2B.2 (At1g08170) CSB-YP871056DOA
CSB-EP871056DOA
CSB-BP871056DOA
CSB-MP871056DOA
CSB-EP871056DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine/threonine-protein kinase-like protein At1g28390 (At1g28390) CSB-YP871057DOA
CSB-EP871057DOA
CSB-BP871057DOA
CSB-MP871057DOA
CSB-EP871057DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana High mobility group B protein 9 (HMGB9) CSB-YP871058DOA
CSB-EP871058DOA
CSB-BP871058DOA
CSB-MP871058DOA
CSB-EP871058DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter B family member 10 (ABCB10), partial CSB-YP871059DOA
CSB-EP871059DOA
CSB-BP871059DOA
CSB-MP871059DOA
CSB-EP871059DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable disease resistance protein At1g63360 (At1g63360), partial CSB-YP871060DOA
CSB-EP871060DOA
CSB-BP871060DOA
CSB-MP871060DOA
CSB-EP871060DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cysteine-rich repeat secretory protein 7 (CRRSP7) CSB-YP871061DOA
CSB-EP871061DOA
CSB-BP871061DOA
CSB-MP871061DOA
CSB-EP871061DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g64100 (At1g64100), partial CSB-YP871062DOA
CSB-EP871062DOA
CSB-BP871062DOA
CSB-MP871062DOA
CSB-EP871062DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Vesicle-associated protein 1-2 (PVA12), partial CSB-YP871063DOA
CSB-EP871063DOA
CSB-BP871063DOA
CSB-MP871063DOA
CSB-EP871063DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein argonaute 2 (AGO2), partial CSB-YP871064DOA
CSB-EP871064DOA
CSB-BP871064DOA
CSB-MP871064DOA
CSB-EP871064DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutathione S-transferase U26 (GSTU26) CSB-YP871065DOA
CSB-EP871065DOA
CSB-BP871065DOA
CSB-MP871065DOA
CSB-EP871065DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glycerol-3-phosphate acyltransferase 1 (GPAT1), partial CSB-YP871066DOA1
CSB-EP871066DOA1
CSB-BP871066DOA1
CSB-MP871066DOA1
CSB-EP871066DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable ADP-ribosylation factor At2g15310 (At2g15310) CSB-YP871067DOA
CSB-EP871067DOA
CSB-BP871067DOA
CSB-MP871067DOA
CSB-EP871067DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative expansin-B2 (EXPB2) CSB-YP871068DOA
CSB-EP871068DOA
CSB-BP871068DOA
CSB-MP871068DOA
CSB-EP871068DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Metal tolerance protein 12 (MTP12), partial CSB-YP871069DOA1
CSB-EP871069DOA1
CSB-BP871069DOA1
CSB-MP871069DOA1
CSB-EP871069DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana WRKY transcription factor 1 (WRKY1) CSB-YP871070DOA
CSB-EP871070DOA
CSB-BP871070DOA
CSB-MP871070DOA
CSB-EP871070DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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