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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Classical arabinogalactan protein 10 (AGP10) CSB-YP878660DOA
CSB-EP878660DOA
CSB-BP878660DOA
CSB-MP878660DOA
CSB-EP878660DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/LRR-repeat protein 21 (FBL21) CSB-YP878661DOA
CSB-EP878661DOA
CSB-BP878661DOA
CSB-MP878661DOA
CSB-EP878661DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative PAP-specific phosphatase, mitochondrial (At4g05090) CSB-YP878662DOA
CSB-EP878662DOA
CSB-BP878662DOA
CSB-MP878662DOA
CSB-EP878662DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GEM-like protein 2 (At4g01600) CSB-YP878663DOA
CSB-EP878663DOA
CSB-BP878663DOA
CSB-MP878663DOA
CSB-EP878663DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g00950 (At4g00950) CSB-YP878664DOA
CSB-EP878664DOA
CSB-BP878664DOA
CSB-MP878664DOA
CSB-EP878664DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At3g59610 (At3g59610), partial CSB-YP878665DOA
CSB-EP878665DOA
CSB-BP878665DOA
CSB-MP878665DOA
CSB-EP878665DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Citrate synthase 5, mitochondrial (CSY5) CSB-YP878666DOA
CSB-EP878666DOA
CSB-BP878666DOA
CSB-MP878666DOA
CSB-EP878666DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Extensin-2 (EXT2), partial CSB-YP878667DOA
CSB-EP878667DOA
CSB-BP878667DOA
CSB-MP878667DOA
CSB-EP878667DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable S-acyltransferase At3g56930 (At3g56930), partial CSB-YP878668DOA1
CSB-EP878668DOA1
CSB-BP878668DOA1
CSB-MP878668DOA1
CSB-EP878668DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CDT1-like protein b (CDT1B) CSB-YP878669DOA
CSB-EP878669DOA
CSB-BP878669DOA
CSB-MP878669DOA
CSB-EP878669DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Heat stress transcription factor A-7b (HSFA7B) CSB-YP878670DOA
CSB-EP878670DOA
CSB-BP878670DOA
CSB-MP878670DOA
CSB-EP878670DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine protease ATG4b (ATG4B) CSB-YP878671DOA
CSB-EP878671DOA
CSB-BP878671DOA
CSB-MP878671DOA
CSB-EP878671DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF035 (ERF035) CSB-YP878672DOA
CSB-EP878672DOA
CSB-BP878672DOA
CSB-MP878672DOA
CSB-EP878672DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative NAC domain-containing protein 61 (NAC061) CSB-YP878673DOA
CSB-EP878673DOA
CSB-BP878673DOA
CSB-MP878673DOA
CSB-EP878673DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Vacuolar iron transporter homolog 3 (At3g43630), partial CSB-YP878674DOA1
CSB-EP878674DOA1
CSB-BP878674DOA1
CSB-MP878674DOA1
CSB-EP878674DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 12 (RH12) CSB-YP878675DOA
CSB-EP878675DOA
CSB-BP878675DOA
CSB-MP878675DOA
CSB-EP878675DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glucan endo-1,3-beta-glucosidase-like protein 1 (At3g58100) CSB-YP878676DOA
CSB-EP878676DOA
CSB-BP878676DOA
CSB-MP878676DOA
CSB-EP878676DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Squamosa promoter-binding-like protein 15 (SPL15) CSB-YP878677DOA
CSB-EP878677DOA
CSB-BP878677DOA
CSB-MP878677DOA
CSB-EP878677DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AP3-complex subunit beta-A (AP3BA), partial CSB-YP878678DOA
CSB-EP878678DOA
CSB-BP878678DOA
CSB-MP878678DOA
CSB-EP878678DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana BTB/POZ domain-containing protein At3g49900 (At3g49900), partial CSB-YP878679DOA
CSB-EP878679DOA
CSB-BP878679DOA
CSB-MP878679DOA
CSB-EP878679DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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