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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Putative non-specific lipid-transfer protein 14 (LTP14) CSB-YP880753DOA
CSB-EP880753DOA
CSB-BP880753DOA
CSB-MP880753DOA
CSB-EP880753DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-glucosidase 42 (BGLU42) CSB-YP880754DOA
CSB-EP880754DOA
CSB-BP880754DOA
CSB-MP880754DOA
CSB-EP880754DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative transcription factor bHLH086 (BHLH86) CSB-YP880755DOA
CSB-EP880755DOA
CSB-BP880755DOA
CSB-MP880755DOA
CSB-EP880755DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At5g45950 (At5g45950) CSB-YP880756DOA
CSB-EP880756DOA
CSB-BP880756DOA
CSB-MP880756DOA
CSB-EP880756DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable long-chain-alcohol O-fatty-acyltransferase 1 (AT1), partial CSB-YP880757DOA
CSB-EP880757DOA
CSB-BP880757DOA
CSB-MP880757DOA
CSB-EP880757DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable long-chain-alcohol O-fatty-acyltransferase 6 (AT6), partial CSB-YP880758DOA
CSB-EP880758DOA
CSB-BP880758DOA
CSB-MP880758DOA
CSB-EP880758DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor TT2 (TT2) CSB-YP880759DOA
CSB-EP880759DOA
CSB-BP880759DOA
CSB-MP880759DOA
CSB-EP880759DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Laccase-17 (LAC17), partial CSB-YP880760DOA
CSB-EP880760DOA
CSB-BP880760DOA
CSB-MP880760DOA
CSB-EP880760DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana High affinity nitrate transporter 2.3 (NRT2.3), partial CSB-YP880761DOA
CSB-EP880761DOA
CSB-BP880761DOA
CSB-MP880761DOA
CSB-EP880761DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable disease resistance RPP8-like protein 4 (RPP8L4), partial CSB-YP880762DOA
CSB-EP880762DOA
CSB-BP880762DOA
CSB-MP880762DOA
CSB-EP880762DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial (SDH2-3) CSB-YP880763DOA
CSB-EP880763DOA
CSB-BP880763DOA
CSB-MP880763DOA
CSB-EP880763DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone acetyltransferase type B catalytic subunit (HAG2) CSB-YP880764DOA
CSB-EP880764DOA
CSB-BP880764DOA
CSB-MP880764DOA
CSB-EP880764DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative FBD-associated F-box protein At5g56560 (At5g56560) CSB-YP880765DOA
CSB-EP880765DOA
CSB-BP880765DOA
CSB-MP880765DOA
CSB-EP880765DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana BTB/POZ and TAZ domain-containing protein 4 (BT4) CSB-YP880766DOA
CSB-EP880766DOA
CSB-BP880766DOA
CSB-MP880766DOA
CSB-EP880766DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxidase 72 (PER72) CSB-YP880767DOA
CSB-EP880767DOA
CSB-BP880767DOA
CSB-MP880767DOA
CSB-EP880767DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g50450 (At5g50450) CSB-YP880768DOA
CSB-EP880768DOA
CSB-BP880768DOA
CSB-MP880768DOA
CSB-EP880768DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g18160 (At5g18160) CSB-YP880769DOA
CSB-EP880769DOA
CSB-BP880769DOA
CSB-MP880769DOA
CSB-EP880769DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g39680 (EMB2744), partial CSB-YP880770DOA
CSB-EP880770DOA
CSB-BP880770DOA
CSB-MP880770DOA
CSB-EP880770DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana BTB/POZ domain-containing protein At5g48800 (At5g48800), partial CSB-YP880771DOA
CSB-EP880771DOA
CSB-BP880771DOA
CSB-MP880771DOA
CSB-EP880771DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative E3 ubiquitin-protein ligase SINA-like 6 (At5g37870) CSB-YP880772DOA
CSB-EP880772DOA
CSB-BP880772DOA
CSB-MP880772DOA
CSB-EP880772DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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