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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g22690 (PCMP-H56), partial CSB-YP888824DOA
CSB-EP888824DOA
CSB-BP888824DOA
CSB-MP888824DOA
CSB-EP888824DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g22670, mitochondrial (At3g22670), partial CSB-YP888825DOA
CSB-EP888825DOA
CSB-BP888825DOA
CSB-MP888825DOA
CSB-EP888825DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At3g14380 (At3g14380), partial CSB-YP888826DOA1
CSB-EP888826DOA1
CSB-BP888826DOA1
CSB-MP888826DOA1
CSB-EP888826DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At3g17710 (At3g17710) CSB-YP888827DOA
CSB-EP888827DOA
CSB-BP888827DOA
CSB-MP888827DOA
CSB-EP888827DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g16610 (PCMP-E91), partial CSB-YP888828DOA
CSB-EP888828DOA
CSB-BP888828DOA
CSB-MP888828DOA
CSB-EP888828DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/LRR-repeat protein At3g16555 (At3g16555) CSB-YP888829DOA
CSB-EP888829DOA
CSB-BP888829DOA
CSB-MP888829DOA
CSB-EP888829DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g17270 (At3g17270) CSB-YP888830DOA
CSB-EP888830DOA
CSB-BP888830DOA
CSB-MP888830DOA
CSB-EP888830DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Linoleate 9S-lipoxygenase 5, chloroplastic (LOX5), partial CSB-YP888831DOA
CSB-EP888831DOA
CSB-BP888831DOA
CSB-MP888831DOA
CSB-EP888831DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutamate synthase 1 [NADH], chloroplastic (GLT1), partial CSB-YP888832DOA
CSB-EP888832DOA
CSB-BP888832DOA
CSB-MP888832DOA
CSB-EP888832DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Rho guanine nucleotide exchange factor 8 (ROPGEF8), partial CSB-YP888833DOA
CSB-EP888833DOA
CSB-BP888833DOA
CSB-MP888833DOA
CSB-EP888833DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ras-related protein RABH1a (RABH1A) CSB-YP888834DOA
CSB-EP888834DOA
CSB-BP888834DOA
CSB-MP888834DOA
CSB-EP888834DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Arabinogalactan peptide 14 (AGP14) CSB-YP888835DOA
CSB-EP888835DOA
CSB-BP888835DOA
CSB-MP888835DOA
CSB-EP888835DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nitrate transporter 1.3 (NRT1.3), partial CSB-YP888836DOA
CSB-EP888836DOA
CSB-BP888836DOA
CSB-MP888836DOA
CSB-EP888836DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxidase 67 (PER67) CSB-YP888837DOA
CSB-EP888837DOA
CSB-BP888837DOA
CSB-MP888837DOA
CSB-EP888837DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uric acid degradation bifunctional protein TTL (TTL) CSB-YP888838DOA
CSB-EP888838DOA
CSB-BP888838DOA
CSB-MP888838DOA
CSB-EP888838DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative terpenoid synthase 16 (TPS16), partial CSB-YP888839DOA
CSB-EP888839DOA
CSB-BP888839DOA
CSB-MP888839DOA
CSB-EP888839DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable F-box protein At5g47300 (At5g47300) CSB-YP888840DOA
CSB-EP888840DOA
CSB-BP888840DOA
CSB-MP888840DOA
CSB-EP888840DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 79B1 (UGT79B1) CSB-YP888841DOA
CSB-EP888841DOA
CSB-BP888841DOA
CSB-MP888841DOA
CSB-EP888841DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome c oxidase subunit 5b-1, mitochondrial (COX5B-1) CSB-YP888842DOA
CSB-EP888842DOA
CSB-BP888842DOA
CSB-MP888842DOA
CSB-EP888842DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing transcription factor FUS3 (FUS3) CSB-YP888843DOA
CSB-EP888843DOA
CSB-BP888843DOA
CSB-MP888843DOA
CSB-EP888843DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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