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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria welshimeri serovar 6b Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP366444LLT
CSB-EP366444LLT
CSB-BP366444LLT
CSB-MP366444LLT
CSB-EP366444LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP366445LLT
CSB-EP366445LLT
CSB-BP366445LLT
CSB-MP366445LLT
CSB-EP366445LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP366446LLT
CSB-EP366446LLT
CSB-BP366446LLT
CSB-MP366446LLT
CSB-EP366446LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP366447LLT
CSB-EP366447LLT
CSB-BP366447LLT
CSB-MP366447LLT
CSB-EP366447LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP366448LLT
CSB-EP366448LLT
CSB-BP366448LLT
CSB-MP366448LLT
CSB-EP366448LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b UPF0316 protein lwe1794 (lwe1794), partial CSB-YP366449LLT1
CSB-EP366449LLT1
CSB-BP366449LLT1
CSB-MP366449LLT1
CSB-EP366449LLT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L19 (rplS) CSB-YP366450LLT
CSB-EP366450LLT
CSB-BP366450LLT
CSB-MP366450LLT
CSB-EP366450LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b UPF0122 protein lwe1821 (lwe1821) CSB-YP366451LLT
CSB-EP366451LLT
CSB-BP366451LLT
CSB-MP366451LLT
CSB-EP366451LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Transcription factor fapR (fapR) CSB-YP366452LLT
CSB-EP366452LLT
CSB-BP366452LLT
CSB-MP366452LLT
CSB-EP366452LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP366453LLT
CSB-EP366453LLT
CSB-BP366453LLT
CSB-MP366453LLT
CSB-EP366453LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit CSB-YP366454LLT
CSB-EP366454LLT
CSB-BP366454LLT
CSB-MP366454LLT
CSB-EP366454LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Aspartate carbamoyltransferase (pyrB) CSB-YP366455LLT
CSB-EP366455LLT
CSB-BP366455LLT
CSB-MP366455LLT
CSB-EP366455LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Lipoprotein signal peptidase (lspA), partial CSB-YP366456LLT1
CSB-EP366456LLT1
CSB-BP366456LLT1
CSB-MP366456LLT1
CSB-EP366456LLT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP366457LLT
CSB-EP366457LLT
CSB-BP366457LLT
CSB-MP366457LLT
CSB-EP366457LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Putative phosphotransferase lwe1885 (lwe1885) CSB-YP366458LLT
CSB-EP366458LLT
CSB-BP366458LLT
CSB-MP366458LLT
CSB-EP366458LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Formate--tetrahydrofolate ligase (fhs), partial CSB-YP366459LLT
CSB-EP366459LLT
CSB-BP366459LLT
CSB-MP366459LLT
CSB-EP366459LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Transcriptional regulator MntR (mntR) CSB-YP366460LLT
CSB-EP366460LLT
CSB-BP366460LLT
CSB-MP366460LLT
CSB-EP366460LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Cell cycle protein GpsB (gpsB) CSB-YP366461LLT
CSB-EP366461LLT
CSB-BP366461LLT
CSB-MP366461LLT
CSB-EP366461LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Holliday junction resolvase recU (recU) CSB-YP366462LLT
CSB-EP366462LLT
CSB-BP366462LLT
CSB-MP366462LLT
CSB-EP366462LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Aspartate 1-decarboxylase (panD) CSB-YP366463LLT
CSB-EP366463LLT
CSB-BP366463LLT
CSB-MP366463LLT
CSB-EP366463LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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