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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium smegmatis Porin MspC (mspC) CSB-YP368044MVX
CSB-EP368044MVX
CSB-BP368044MVX
CSB-MP368044MVX
CSB-EP368044MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Response regulator mprA (mprA) CSB-YP368045MVX
CSB-EP368045MVX
CSB-BP368045MVX
CSB-MP368045MVX
CSB-EP368045MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Carboxylate-amine ligase MSMEG_5590 (MSMEG_5590) CSB-YP368046MVX
CSB-EP368046MVX
CSB-BP368046MVX
CSB-MP368046MVX
CSB-EP368046MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Alpha-keto-acid decarboxylase (kdc), partial CSB-YP368047MVX
CSB-EP368047MVX
CSB-BP368047MVX
CSB-MP368047MVX
CSB-EP368047MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Putative thiosulfate sulfurtransferase (MSMEG_5789) CSB-YP368048MVX
CSB-EP368048MVX
CSB-BP368048MVX
CSB-MP368048MVX
CSB-EP368048MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis KsdD-like steroid dehydrogenase MSMEG_5835 (MSMEG_5835), partial CSB-YP368049MVX
CSB-EP368049MVX
CSB-BP368049MVX
CSB-MP368049MVX
CSB-EP368049MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Putative succinate-semialdehyde dehydrogenase [NADP (+)], partial CSB-YP368050MVX
CSB-EP368050MVX
CSB-BP368050MVX
CSB-MP368050MVX
CSB-EP368050MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD), partial CSB-YP368051MVX
CSB-BP368051MVX
CSB-MP368051MVX
CSB-EP368051MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis HTH-type transcriptional repressor KstR (kstR) CSB-YP368052MVX
CSB-EP368052MVX
CSB-BP368052MVX
CSB-MP368052MVX
CSB-EP368052MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis 30S ribosomal protein S18 1 (rpsR1) CSB-YP368053MVX
CSB-EP368053MVX
CSB-BP368053MVX
CSB-MP368053MVX
CSB-EP368053MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Uncharacterized tRNA/rRNA methyltransferase MSMEG_6073 (MSMEG_6073) CSB-YP368054MVX
CSB-EP368054MVX
CSB-BP368054MVX
CSB-MP368054MVX
CSB-EP368054MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Probable cold shock protein A (cspA) CSB-YP368055MVX
CSB-EP368055MVX
CSB-BP368055MVX
CSB-MP368055MVX
CSB-EP368055MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Histidine-specific methyltransferase EgtD (egtD) CSB-YP368056MVX
CSB-EP368056MVX
CSB-BP368056MVX
CSB-MP368056MVX
CSB-EP368056MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA (gshA) CSB-YP368057MVX
CSB-EP368057MVX
CSB-BP368057MVX
CSB-MP368057MVX
CSB-EP368057MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Putative phenylalanine aminotransferase (pat) CSB-YP368058MVX
CSB-EP368058MVX
CSB-BP368058MVX
CSB-MP368058MVX
CSB-EP368058MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Arabinofuranosyltransferase AftA (aftA), partial CSB-YP368059MVX
CSB-EP368059MVX
CSB-BP368059MVX
CSB-MP368059MVX
CSB-EP368059MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Prephenate dehydratase (pheA) CSB-YP368060MVX
CSB-EP368060MVX
CSB-BP368060MVX
CSB-MP368060MVX
CSB-EP368060MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Ferritin BfrB (bfrB) CSB-YP368061MVX
CSB-EP368061MVX
CSB-BP368061MVX
CSB-MP368061MVX
CSB-EP368061MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis FAD-containing monooxygenase EthA (ethA) CSB-YP368062MVX
CSB-EP368062MVX
CSB-BP368062MVX
CSB-MP368062MVX
CSB-EP368062MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Trehalose synthase/amylase TreS (treS), partial CSB-YP368063MVX
CSB-EP368063MVX
CSB-BP368063MVX
CSB-MP368063MVX
CSB-EP368063MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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