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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium smegmatis Chorismate synthase (aroC) CSB-YP368004MVX
CSB-EP368004MVX
CSB-BP368004MVX
CSB-MP368004MVX
CSB-EP368004MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP368005MVX
CSB-EP368005MVX
CSB-BP368005MVX
CSB-MP368005MVX
CSB-EP368005MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis UvrABC system protein C (uvrC), partial CSB-YP368006MVX
CSB-EP368006MVX
CSB-BP368006MVX
CSB-MP368006MVX
CSB-EP368006MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Putative sporulation transcription regulator WhiA (whiA) CSB-YP368007MVX
CSB-EP368007MVX
CSB-BP368007MVX
CSB-MP368007MVX
CSB-EP368007MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP368008MVX
CSB-EP368008MVX
CSB-BP368008MVX
CSB-MP368008MVX
CSB-EP368008MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Biotin synthase (bioB) CSB-YP368009MVX
CSB-EP368009MVX
CSB-BP368009MVX
CSB-MP368009MVX
CSB-EP368009MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP368010MVX
CSB-EP368010MVX
CSB-BP368010MVX
CSB-MP368010MVX
CSB-EP368010MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Anthranilate synthase component 1 (trpE), partial CSB-YP368011MVX
CSB-EP368011MVX
CSB-BP368011MVX
CSB-MP368011MVX
CSB-EP368011MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Carboxylate-amine ligase MSMEG_3326 (MSMEG_3326) CSB-YP368012MVX
CSB-EP368012MVX
CSB-BP368012MVX
CSB-MP368012MVX
CSB-EP368012MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Urease subunit alpha (ureC), partial CSB-YP368013MVX
CSB-EP368013MVX
CSB-BP368013MVX
CSB-MP368013MVX
CSB-EP368013MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Protein translocase subunit SecA 2 (secA2), partial CSB-YP368014MVX
CSB-EP368014MVX
CSB-BP368014MVX
CSB-MP368014MVX
CSB-EP368014MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX (lysX), partial CSB-YP368015MVX
CSB-EP368015MVX
CSB-BP368015MVX
CSB-MP368015MVX
CSB-EP368015MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Pup--protein ligase (pafA) CSB-YP368016MVX
CSB-EP368016MVX
CSB-BP368016MVX
CSB-MP368016MVX
CSB-EP368016MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP368017MVX
CSB-EP368017MVX
CSB-BP368017MVX
CSB-MP368017MVX
CSB-EP368017MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis 4-hydroxy-2-oxovalerate aldolase 1 (MSMEG_4150) CSB-YP368018MVX
CSB-EP368018MVX
CSB-BP368018MVX
CSB-MP368018MVX
CSB-EP368018MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Undecaprenyl-diphosphatase (uppP), partial CSB-YP368019MVX
CSB-EP368019MVX
CSB-BP368019MVX
CSB-MP368019MVX
CSB-EP368019MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP368020MVX
CSB-EP368020MVX
CSB-BP368020MVX
CSB-MP368020MVX
CSB-EP368020MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Protein MraZ (mraZ) CSB-YP368021MVX
CSB-EP368021MVX
CSB-BP368021MVX
CSB-MP368021MVX
CSB-EP368021MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Polyprenol-phosphate-mannose-dependent alpha- (1-2)-phosphatidylinositol mannoside mannosyltransferase, partial CSB-YP368022MVX
CSB-EP368022MVX
CSB-BP368022MVX
CSB-MP368022MVX
CSB-EP368022MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Cobalamin synthase (cobS), partial CSB-YP368023MVX1
CSB-EP368023MVX1
CSB-BP368023MVX1
CSB-MP368023MVX1
CSB-EP368023MVX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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