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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium smegmatis 50S ribosomal protein L9 (rplI) CSB-YP368064MVX
CSB-EP368064MVX
CSB-BP368064MVX
CSB-MP368064MVX
CSB-EP368064MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis 30S ribosomal protein S6 (rpsF) CSB-YP368065MVX
CSB-EP368065MVX
CSB-BP368065MVX
CSB-MP368065MVX
CSB-EP368065MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP368066MVX
CSB-EP368066MVX
CSB-BP368066MVX
CSB-MP368066MVX
CSB-EP368066MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Serine--tRNA ligase (serS) CSB-YP368067BOM
CSB-EP368067BOM
CSB-BP368067BOM
CSB-MP368067BOM
CSB-EP368067BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Recombination protein RecR (recR) CSB-YP368068BOM
CSB-EP368068BOM
CSB-BP368068BOM
CSB-MP368068BOM
CSB-EP368068BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP368069BOM
CSB-EP368069BOM
CSB-BP368069BOM
CSB-MP368069BOM
CSB-EP368069BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis DNA integrity scanning protein DisA (disA) CSB-YP368070BOM
CSB-EP368070BOM
CSB-BP368070BOM
CSB-MP368070BOM
CSB-EP368070BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Glutamate--tRNA ligase (gltX) CSB-YP368071BOM
CSB-EP368071BOM
CSB-BP368071BOM
CSB-MP368071BOM
CSB-EP368071BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L1 (rplA) CSB-YP368072BOM
CSB-EP368072BOM
CSB-BP368072BOM
CSB-MP368072BOM
CSB-EP368072BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP368073BOM
CSB-EP368073BOM
CSB-BP368073BOM
CSB-MP368073BOM
CSB-EP368073BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L3 (rplC) CSB-YP368074BOM
CSB-EP368074BOM
CSB-BP368074BOM
CSB-MP368074BOM
CSB-EP368074BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L2 (rplB) CSB-YP368075BOM
CSB-EP368075BOM
CSB-BP368075BOM
CSB-MP368075BOM
CSB-EP368075BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 30S ribosomal protein S3 (rpsC) CSB-YP368076BOM
CSB-EP368076BOM
CSB-BP368076BOM
CSB-MP368076BOM
CSB-EP368076BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 30S ribosomal protein S17 (rpsQ) CSB-YP368077BOM
CSB-EP368077BOM
CSB-BP368077BOM
CSB-MP368077BOM
CSB-EP368077BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP368078BOM
CSB-EP368078BOM
CSB-BP368078BOM
CSB-MP368078BOM
CSB-EP368078BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 30S ribosomal protein S8 (rpsH) CSB-YP368079BOM
CSB-EP368079BOM
CSB-BP368079BOM
CSB-MP368079BOM
CSB-EP368079BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L30 (rpmD) CSB-YP368080BOM
CSB-EP368080BOM
CSB-BP368080BOM
CSB-MP368080BOM
CSB-EP368080BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Translation initiation factor IF-1 (infA) CSB-YP368081BOM
CSB-EP368081BOM
CSB-BP368081BOM
CSB-MP368081BOM
CSB-EP368081BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP368082BOM
CSB-EP368082BOM
CSB-BP368082BOM
CSB-MP368082BOM
CSB-EP368082BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP368083BOM
CSB-EP368083BOM
CSB-BP368083BOM
CSB-MP368083BOM
CSB-EP368083BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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