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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis HTH-type transcriptional regulator CzcR (czcR) CSB-YP368084BOM
CSB-EP368084BOM
CSB-BP368084BOM
CSB-MP368084BOM
CSB-EP368084BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP368085BOM
CSB-EP368085BOM
CSB-BP368085BOM
CSB-MP368085BOM
CSB-EP368085BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Undecaprenyl-diphosphatase 1 (uppP1), partial CSB-YP368086BOM1
CSB-EP368086BOM1
CSB-BP368086BOM1
CSB-MP368086BOM1
CSB-EP368086BOM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP368087BOM
CSB-EP368087BOM
CSB-BP368087BOM
CSB-MP368087BOM
CSB-EP368087BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis DNA ligase (ligA), partial CSB-YP368088BOM
CSB-EP368088BOM
CSB-BP368088BOM
CSB-MP368088BOM
CSB-EP368088BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Methylthioribose-1-phosphate isomerase 1 (mtnA1) CSB-YP368089BOM
CSB-EP368089BOM
CSB-BP368089BOM
CSB-MP368089BOM
CSB-EP368089BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Arginine deiminase (arcA) CSB-YP368090BOM
CSB-EP368090BOM
CSB-BP368090BOM
CSB-MP368090BOM
CSB-EP368090BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Thiamine-phosphate synthase (thiE) CSB-YP368091BOM
CSB-EP368091BOM
CSB-BP368091BOM
CSB-MP368091BOM
CSB-EP368091BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP368092BOM
CSB-EP368092BOM
CSB-BP368092BOM
CSB-MP368092BOM
CSB-EP368092BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0374 protein BALH_0460 (BALH_0460) CSB-YP368093BOM
CSB-EP368093BOM
CSB-BP368093BOM
CSB-MP368093BOM
CSB-EP368093BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis D-ribose pyranase (rbsD) CSB-YP368094BOM
CSB-EP368094BOM
CSB-BP368094BOM
CSB-MP368094BOM
CSB-EP368094BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Potassium-transporting ATPase B chain (kdpB), partial CSB-YP368095BOM1
CSB-EP368095BOM1
CSB-BP368095BOM1
CSB-MP368095BOM1
CSB-EP368095BOM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Putative 3-methyladenine DNA glycosylase (BALH_0786) CSB-YP368096BOM
CSB-EP368096BOM
CSB-BP368096BOM
CSB-MP368096BOM
CSB-EP368096BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Glycerol kinase (glpK) CSB-YP368097BOM
CSB-EP368097BOM
CSB-BP368097BOM
CSB-MP368097BOM
CSB-EP368097BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP368098BOM
CSB-EP368098BOM
CSB-BP368098BOM
CSB-MP368098BOM
CSB-EP368098BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0477 protein BALH_1087 (BALH_1087) CSB-YP368099BOM
CSB-EP368099BOM
CSB-BP368099BOM
CSB-MP368099BOM
CSB-EP368099BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Tryptophan synthase beta chain (trpB) CSB-YP368100BOM
CSB-EP368100BOM
CSB-BP368100BOM
CSB-MP368100BOM
CSB-EP368100BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Tryptophan synthase alpha chain (trpA) CSB-YP368101BOM
CSB-EP368101BOM
CSB-BP368101BOM
CSB-MP368101BOM
CSB-EP368101BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Phosphonoacetaldehyde hydrolase (phnX) CSB-YP368102BOM
CSB-EP368102BOM
CSB-BP368102BOM
CSB-MP368102BOM
CSB-EP368102BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 7-cyano-7-deazaguanine synthase (queC) CSB-YP368103BOM
CSB-EP368103BOM
CSB-BP368103BOM
CSB-MP368103BOM
CSB-EP368103BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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