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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Gramella forsetii 50S ribosomal protein L27 (rpmA) CSB-YP367567GEQ
CSB-EP367567GEQ
CSB-BP367567GEQ
CSB-MP367567GEQ
CSB-EP367567GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine reproductive and respiratory syndrome virus Glycoprotein 2a (GP2a), partial CSB-YP367568PYZ1
CSB-EP367568PYZ1
CSB-BP367568PYZ1
CSB-EP367568PYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine reproductive and respiratory syndrome virus Glycoprotein 5 (GP5), partial CSB-YP367569PYZ1
CSB-BP367569PYZ1
CSB-MP367569PYZ1
CSB-EP367569PYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine reproductive and respiratory syndrome virus Nucleoprotein (N) CSB-YP367570PYZ
CSB-EP367570PYZ
CSB-BP367570PYZ
CSB-MP367570PYZ
CSB-EP367570PYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 30 (CRRSP30) CSB-YP367571DOA
CSB-EP367571DOA
CSB-BP367571DOA
CSB-MP367571DOA
CSB-EP367571DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Heparan-sulfate 6-O-sulfotransferase 1-B (hs6st1b), partial CSB-YP367572DIL
CSB-EP367572DIL
CSB-BP367572DIL
CSB-MP367572DIL
CSB-EP367572DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bear canyon virus Pre-glycoprotein polyprotein GP complex (GPC), partial CSB-YP367573BOW1
CSB-EP367573BOW1
CSB-BP367573BOW1
CSB-MP367573BOW1
CSB-EP367573BOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP367574MOP
CSB-EP367574MOP
CSB-BP367574MOP
CSB-MP367574MOP
CSB-EP367574MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans UPF0233 membrane protein MUL_0015 (MUL_0015), partial CSB-YP367575MOP1
CSB-EP367575MOP1
CSB-BP367575MOP1
CSB-MP367575MOP1
CSB-EP367575MOP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans 50S ribosomal protein L9 (rplI) CSB-YP367576MOP
CSB-EP367576MOP
CSB-BP367576MOP
CSB-MP367576MOP
CSB-EP367576MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Pantothenate kinase (coaA) CSB-YP367577MOP
CSB-EP367577MOP
CSB-BP367577MOP
CSB-MP367577MOP
CSB-EP367577MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase (mhpB) CSB-YP367578MOP
CSB-EP367578MOP
CSB-BP367578MOP
CSB-MP367578MOP
CSB-EP367578MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Copper-sensing transcriptional repressor CsoR (csoR) CSB-YP367579MOP
CSB-EP367579MOP
CSB-BP367579MOP
CSB-MP367579MOP
CSB-EP367579MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Deoxycytidine triphosphate deaminase (dcd) CSB-YP367580MOP
CSB-EP367580MOP
CSB-BP367580MOP
CSB-MP367580MOP
CSB-EP367580MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP367581MOP
CSB-EP367581MOP
CSB-BP367581MOP
CSB-MP367581MOP
CSB-EP367581MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Demethylmenaquinone methyltransferase (ubiE) CSB-YP367582MOP
CSB-EP367582MOP
CSB-BP367582MOP
CSB-MP367582MOP
CSB-EP367582MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Putative S-adenosyl-L-methionine-dependent methyltransferase MUL_0706 (MUL_0706) CSB-YP367583MOP
CSB-EP367583MOP
CSB-BP367583MOP
CSB-MP367583MOP
CSB-EP367583MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP367584MOP
CSB-EP367584MOP
CSB-BP367584MOP
CSB-MP367584MOP
CSB-EP367584MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP367585MOP
CSB-EP367585MOP
CSB-BP367585MOP
CSB-MP367585MOP
CSB-EP367585MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans 30S ribosomal protein S10 (rpsJ) CSB-YP367586MOP
CSB-EP367586MOP
CSB-BP367586MOP
CSB-MP367586MOP
CSB-EP367586MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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