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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis 50S ribosomal protein L22 (rplV) CSB-YP369905BOM
CSB-EP369905BOM
CSB-BP369905BOM
CSB-MP369905BOM
CSB-EP369905BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L14 (rplN) CSB-YP369906BOM
CSB-EP369906BOM
CSB-BP369906BOM
CSB-MP369906BOM
CSB-EP369906BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L24 (rplX) CSB-YP369907BOM
CSB-EP369907BOM
CSB-BP369907BOM
CSB-MP369907BOM
CSB-EP369907BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 30S ribosomal protein S5 (rpsE) CSB-YP369908BOM
CSB-EP369908BOM
CSB-BP369908BOM
CSB-MP369908BOM
CSB-EP369908BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Adenylate kinase (adk) CSB-YP369909BOM
CSB-EP369909BOM
CSB-BP369909BOM
CSB-MP369909BOM
CSB-EP369909BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 30S ribosomal protein S11 (rpsK) CSB-YP369910BOM
CSB-EP369910BOM
CSB-BP369910BOM
CSB-MP369910BOM
CSB-EP369910BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L17 (rplQ) CSB-YP369911BOM
CSB-EP369911BOM
CSB-BP369911BOM
CSB-MP369911BOM
CSB-EP369911BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP369912BOM
CSB-EP369912BOM
CSB-BP369912BOM
CSB-MP369912BOM
CSB-EP369912BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 30S ribosomal protein S9 (rpsI) CSB-YP369913BOM
CSB-EP369913BOM
CSB-BP369913BOM
CSB-MP369913BOM
CSB-EP369913BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis DEAD-box ATP-dependent RNA helicase CshA (cshA), partial CSB-YP369914BOM
CSB-EP369914BOM
CSB-BP369914BOM
CSB-MP369914BOM
CSB-EP369914BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 60 kDa chaperonin (groL), partial CSB-YP369915BOM
CSB-EP369915BOM
CSB-BP369915BOM
CSB-MP369915BOM
CSB-EP369915BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP369916BOM
CSB-EP369916BOM
CSB-BP369916BOM
CSB-MP369916BOM
CSB-EP369916BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP369917BOM
CSB-EP369917BOM
CSB-BP369917BOM
CSB-MP369917BOM
CSB-EP369917BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP369918BOM
CSB-EP369918BOM
CSB-BP369918BOM
CSB-MP369918BOM
CSB-EP369918BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Ferredoxin--NADP reductase 1 (BALH_0343) CSB-YP369919BOM
CSB-EP369919BOM
CSB-BP369919BOM
CSB-MP369919BOM
CSB-EP369919BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Hydroxyethylthiazole kinase (thiM) CSB-YP369920BOM
CSB-EP369920BOM
CSB-BP369920BOM
CSB-MP369920BOM
CSB-EP369920BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0435 protein BALH_0406 (BALH_0406) CSB-YP369921BOM
CSB-EP369921BOM
CSB-BP369921BOM
CSB-MP369921BOM
CSB-EP369921BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0229 protein BALH_0490 (BALH_0490) CSB-YP369922BOM
CSB-EP369922BOM
CSB-BP369922BOM
CSB-MP369922BOM
CSB-EP369922BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Thiazole synthase (thiG) CSB-YP369923BOM
CSB-EP369923BOM
CSB-BP369923BOM
CSB-MP369923BOM
CSB-EP369923BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0342 protein BALH_0781 (BALH_0781) CSB-YP369924BOM
CSB-EP369924BOM
CSB-BP369924BOM
CSB-MP369924BOM
CSB-EP369924BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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