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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP369965BOM
CSB-EP369965BOM
CSB-BP369965BOM
CSB-MP369965BOM
CSB-EP369965BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit CSB-YP369966BOM
CSB-EP369966BOM
CSB-BP369966BOM
CSB-MP369966BOM
CSB-EP369966BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Aspartate carbamoyltransferase (pyrB) CSB-YP369967BOM
CSB-EP369967BOM
CSB-BP369967BOM
CSB-MP369967BOM
CSB-EP369967BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase 2 CSB-YP369968BOM
CSB-EP369968BOM
CSB-BP369968BOM
CSB-MP369968BOM
CSB-EP369968BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP369969BOM
CSB-EP369969BOM
CSB-BP369969BOM
CSB-MP369969BOM
CSB-EP369969BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0348 protein BALH_3554 (BALH_3554) CSB-YP369970BOM
CSB-EP369970BOM
CSB-BP369970BOM
CSB-MP369970BOM
CSB-EP369970BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Phosphopantetheine adenylyltransferase (coaD) CSB-YP369971BOM
CSB-EP369971BOM
CSB-BP369971BOM
CSB-MP369971BOM
CSB-EP369971BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Heme A synthase (ctaA), partial CSB-YP369972BOM
CSB-EP369972BOM
CSB-BP369972BOM
CSB-MP369972BOM
CSB-EP369972BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0358 protein BALH_3577 (BALH_3577) CSB-YP369973BOM
CSB-EP369973BOM
CSB-BP369973BOM
CSB-MP369973BOM
CSB-EP369973BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Protein-glutamine gamma-glutamyltransferase (tgl) CSB-YP369974BOM
CSB-EP369974BOM
CSB-BP369974BOM
CSB-MP369974BOM
CSB-EP369974BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Methylthioribose-1-phosphate isomerase 2 (mtnA2) CSB-YP369975BOM
CSB-EP369975BOM
CSB-BP369975BOM
CSB-MP369975BOM
CSB-EP369975BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP369976BOM
CSB-EP369976BOM
CSB-BP369976BOM
CSB-MP369976BOM
CSB-EP369976BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Glucosamine-6-phosphate deaminase (nagB) CSB-YP369977BOM
CSB-EP369977BOM
CSB-BP369977BOM
CSB-MP369977BOM
CSB-EP369977BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Anti-sigma F factor (spoIIAB) CSB-YP369978BOM
CSB-EP369978BOM
CSB-BP369978BOM
CSB-MP369978BOM
CSB-EP369978BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Biotin synthase (bioB) CSB-YP369979BOM
CSB-EP369979BOM
CSB-BP369979BOM
CSB-MP369979BOM
CSB-EP369979BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP369980BOM
CSB-EP369980BOM
CSB-BP369980BOM
CSB-MP369980BOM
CSB-EP369980BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP369981BOM
CSB-EP369981BOM
CSB-BP369981BOM
CSB-MP369981BOM
CSB-EP369981BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP369982BOM
CSB-EP369982BOM
CSB-BP369982BOM
CSB-MP369982BOM
CSB-EP369982BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Probable endonuclease 4 (nfo) CSB-YP369983BOM
CSB-EP369983BOM
CSB-BP369983BOM
CSB-MP369983BOM
CSB-EP369983BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Putative phosphotransferase BALH_3887 (BALH_3887) CSB-YP369984BOM
CSB-EP369984BOM
CSB-BP369984BOM
CSB-MP369984BOM
CSB-EP369984BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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