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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella amazonensis Flagellar P-ring protein (flgI) CSB-YP376428STK
CSB-EP376428STK
CSB-BP376428STK
CSB-MP376428STK
CSB-EP376428STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Histidine--tRNA ligase (hisS) CSB-YP376429STK
CSB-EP376429STK
CSB-BP376429STK
CSB-MP376429STK
CSB-EP376429STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP376430STK
CSB-EP376430STK
CSB-BP376430STK
CSB-MP376430STK
CSB-EP376430STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP376431STK
CSB-EP376431STK
CSB-BP376431STK
CSB-MP376431STK
CSB-EP376431STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Protein grpE (grpE) CSB-YP376432STK
CSB-EP376432STK
CSB-BP376432STK
CSB-MP376432STK
CSB-EP376432STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0235 protein Sama_2480 (Sama_2480) CSB-YP376433STK
CSB-EP376433STK
CSB-BP376433STK
CSB-MP376433STK
CSB-EP376433STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Probable Fe (2+)-trafficking protein CSB-YP376434STK
CSB-EP376434STK
CSB-BP376434STK
CSB-MP376434STK
CSB-EP376434STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Glutamate 5-kinase (proB) CSB-YP376435STK
CSB-EP376435STK
CSB-BP376435STK
CSB-MP376435STK
CSB-EP376435STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0234 protein Sama_2557 (Sama_2557) CSB-YP376436STK
CSB-EP376436STK
CSB-BP376436STK
CSB-MP376436STK
CSB-EP376436STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP376437STK
CSB-EP376437STK
CSB-BP376437STK
CSB-MP376437STK
CSB-EP376437STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Probable rRNA maturation factor (Sama_2577) CSB-YP376438STK
CSB-EP376438STK
CSB-BP376438STK
CSB-MP376438STK
CSB-EP376438STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0250 protein Sama_2593 (Sama_2593) CSB-YP376439STK
CSB-EP376439STK
CSB-BP376439STK
CSB-MP376439STK
CSB-EP376439STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Octanoyltransferase (lipB) CSB-YP376440STK
CSB-EP376440STK
CSB-BP376440STK
CSB-MP376440STK
CSB-EP376440STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP376441STK
CSB-EP376441STK
CSB-BP376441STK
CSB-MP376441STK
CSB-EP376441STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP376442STK
CSB-EP376442STK
CSB-BP376442STK
CSB-MP376442STK
CSB-EP376442STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis S-adenosylmethionine synthase (metK) CSB-YP376443STK
CSB-EP376443STK
CSB-BP376443STK
CSB-MP376443STK
CSB-EP376443STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP376444STK
CSB-EP376444STK
CSB-BP376444STK
CSB-MP376444STK
CSB-EP376444STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 50S ribosomal protein L27 (rpmA) CSB-YP376445STK
CSB-EP376445STK
CSB-BP376445STK
CSB-MP376445STK
CSB-EP376445STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Adenylyl-sulfate kinase (cysC) CSB-YP376446STK
CSB-EP376446STK
CSB-BP376446STK
CSB-MP376446STK
CSB-EP376446STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Protein hfq (hfq) CSB-YP376447STK
CSB-EP376447STK
CSB-BP376447STK
CSB-MP376447STK
CSB-EP376447STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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