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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella amazonensis Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP376368STK
CSB-EP376368STK
CSB-BP376368STK
CSB-MP376368STK
CSB-EP376368STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0231 protein Sama_0645 (Sama_0645) CSB-YP376369STK
CSB-EP376369STK
CSB-BP376369STK
CSB-MP376369STK
CSB-EP376369STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Bifunctional protein HldE (hldE) CSB-YP376370STK
CSB-EP376370STK
CSB-BP376370STK
CSB-MP376370STK
CSB-EP376370STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP376371STK
CSB-EP376371STK
CSB-BP376371STK
CSB-MP376371STK
CSB-EP376371STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Pantothenate synthetase (panC) CSB-YP376372STK
CSB-EP376372STK
CSB-BP376372STK
CSB-MP376372STK
CSB-EP376372STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Sugar fermentation stimulation protein homolog (sfsA) CSB-YP376373STK
CSB-EP376373STK
CSB-BP376373STK
CSB-MP376373STK
CSB-EP376373STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP376374STK
CSB-EP376374STK
CSB-BP376374STK
CSB-MP376374STK
CSB-EP376374STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP376375STK
CSB-EP376375STK
CSB-BP376375STK
CSB-MP376375STK
CSB-EP376375STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S16 (rpsP) CSB-YP376376STK
CSB-EP376376STK
CSB-BP376376STK
CSB-MP376376STK
CSB-EP376376STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Hydroxylamine reductase (hcp), partial CSB-YP376377STK
CSB-EP376377STK
CSB-BP376377STK
CSB-MP376377STK
CSB-EP376377STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Lipoprotein signal peptidase (lspA), partial CSB-YP376378STK1
CSB-EP376378STK1
CSB-BP376378STK1
CSB-MP376378STK1
CSB-EP376378STK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Phosphoglucosamine mutase 1 (glmM1) CSB-YP376379STK
CSB-EP376379STK
CSB-BP376379STK
CSB-MP376379STK
CSB-EP376379STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosome maturation factor RimP (rimP) CSB-YP376380STK
CSB-EP376380STK
CSB-BP376380STK
CSB-MP376380STK
CSB-EP376380STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S15 (rpsO) CSB-YP376381STK
CSB-EP376381STK
CSB-BP376381STK
CSB-MP376381STK
CSB-EP376381STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Deoxyribose-phosphate aldolase (deoC) CSB-YP376382STK
CSB-EP376382STK
CSB-BP376382STK
CSB-MP376382STK
CSB-EP376382STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Chaperone protein TorD (torD) CSB-YP376383STK
CSB-EP376383STK
CSB-BP376383STK
CSB-MP376383STK
CSB-EP376383STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Transcriptional repressor NrdR (nrdR) CSB-YP376384STK
CSB-EP376384STK
CSB-BP376384STK
CSB-MP376384STK
CSB-EP376384STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP376385STK
CSB-EP376385STK
CSB-BP376385STK
CSB-MP376385STK
CSB-EP376385STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP376386STK
CSB-EP376386STK
CSB-BP376386STK
CSB-MP376386STK
CSB-EP376386STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP376387STK
CSB-EP376387STK
CSB-BP376387STK
CSB-MP376387STK
CSB-EP376387STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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