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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella amazonensis 50S ribosomal protein L21 (rplU) CSB-YP377108STK
CSB-EP377108STK
CSB-BP377108STK
CSB-MP377108STK
CSB-EP377108STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Lysine--tRNA ligase (lysS) CSB-YP377109STK
CSB-EP377109STK
CSB-BP377109STK
CSB-MP377109STK
CSB-EP377109STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP377110STK
CSB-EP377110STK
CSB-BP377110STK
CSB-MP377110STK
CSB-EP377110STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis DNA mismatch repair protein mutL (mutL), partial CSB-YP377111STK
CSB-EP377111STK
CSB-BP377111STK
CSB-MP377111STK
CSB-EP377111STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 50S ribosomal protein L9 (rplI) CSB-YP377112STK
CSB-EP377112STK
CSB-BP377112STK
CSB-MP377112STK
CSB-EP377112STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S9 (rpsI) CSB-YP377113STK
CSB-EP377113STK
CSB-BP377113STK
CSB-MP377113STK
CSB-EP377113STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 50S ribosomal protein L13 (rplM) CSB-YP377114STK
CSB-EP377114STK
CSB-BP377114STK
CSB-MP377114STK
CSB-EP377114STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 10 kDa chaperonin (groS) CSB-YP377115STK
CSB-EP377115STK
CSB-BP377115STK
CSB-MP377115STK
CSB-EP377115STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Formate--tetrahydrofolate ligase (fhs), partial CSB-YP377116STK
CSB-EP377116STK
CSB-BP377116STK
CSB-MP377116STK
CSB-EP377116STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP377117STK1
CSB-EP377117STK1
CSB-BP377117STK1
CSB-MP377117STK1
CSB-EP377117STK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis DNA-binding protein fis (fis) CSB-YP377118STK
CSB-EP377118STK
CSB-BP377118STK
CSB-MP377118STK
CSB-EP377118STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Catalase-peroxidase 1 (katG1), partial CSB-YP377119STK
CSB-EP377119STK
CSB-BP377119STK
CSB-MP377119STK
CSB-EP377119STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ketol-acid reductoisomerase (ilvC) CSB-YP377120STK
CSB-EP377120STK
CSB-BP377120STK
CSB-MP377120STK
CSB-EP377120STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0102 protein Sama_3355 (Sama_3355) CSB-YP377121STK
CSB-EP377121STK
CSB-BP377121STK
CSB-MP377121STK
CSB-EP377121STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 3-dehydroquinate synthase (aroB) CSB-YP377122STK
CSB-EP377122STK
CSB-BP377122STK
CSB-MP377122STK
CSB-EP377122STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Shikimate kinase (aroK) CSB-YP377123STK
CSB-EP377123STK
CSB-BP377123STK
CSB-MP377123STK
CSB-EP377123STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0761 membrane protein Sama_3404 (Sama_3404), partial CSB-YP377124STK1
CSB-EP377124STK1
CSB-BP377124STK1
CSB-MP377124STK1
CSB-EP377124STK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP377125STK
CSB-EP377125STK
CSB-BP377125STK
CSB-MP377125STK
CSB-EP377125STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis LexA repressor (lexA) CSB-YP377126STK
CSB-EP377126STK
CSB-BP377126STK
CSB-MP377126STK
CSB-EP377126STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP377127STK
CSB-EP377127STK
CSB-BP377127STK
CSB-MP377127STK
CSB-EP377127STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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