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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella amazonensis Asparagine--tRNA ligase (asnS) CSB-YP377068STK
CSB-EP377068STK
CSB-BP377068STK
CSB-MP377068STK
CSB-EP377068STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Succinylglutamate desuccinylase (astE) CSB-YP377069STK
CSB-EP377069STK
CSB-BP377069STK
CSB-MP377069STK
CSB-EP377069STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP377070STK
CSB-EP377070STK
CSB-BP377070STK
CSB-MP377070STK
CSB-EP377070STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Threonine--tRNA ligase (thrS), partial CSB-YP377071STK
CSB-EP377071STK
CSB-BP377071STK
CSB-MP377071STK
CSB-EP377071STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Protein CrcB homolog (crcB), partial CSB-YP377072STK1
CSB-EP377072STK1
CSB-BP377072STK1
CSB-MP377072STK1
CSB-EP377072STK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Serine--tRNA ligase (serS) CSB-YP377073STK
CSB-EP377073STK
CSB-BP377073STK
CSB-MP377073STK
CSB-EP377073STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Electron transport complex protein RnfB (rnfB) CSB-YP377074STK
CSB-EP377074STK
CSB-BP377074STK
CSB-MP377074STK
CSB-EP377074STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Electron transport complex protein RnfD (rnfD), partial CSB-YP377075STK
CSB-EP377075STK
CSB-BP377075STK
CSB-MP377075STK
CSB-EP377075STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP377076STK
CSB-EP377076STK
CSB-BP377076STK
CSB-MP377076STK
CSB-EP377076STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA (mo5U34)-methyltransferase CSB-YP377077STK
CSB-EP377077STK
CSB-BP377077STK
CSB-MP377077STK
CSB-EP377077STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribonuclease H (rnhA) CSB-YP377078STK
CSB-EP377078STK
CSB-BP377078STK
CSB-MP377078STK
CSB-EP377078STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Na (+)/H (+) antiporter nhaB, partial CSB-YP377079STK
CSB-EP377079STK
CSB-BP377079STK
CSB-MP377079STK
CSB-EP377079STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0745 protein Sama_1929 (Sama_1929) CSB-YP377080STK
CSB-EP377080STK
CSB-BP377080STK
CSB-MP377080STK
CSB-EP377080STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP377081STK
CSB-EP377081STK
CSB-BP377081STK
CSB-MP377081STK
CSB-EP377081STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP377082STK
CSB-EP377082STK
CSB-BP377082STK
CSB-MP377082STK
CSB-EP377082STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Uridine kinase (udk) CSB-YP377083STK
CSB-EP377083STK
CSB-BP377083STK
CSB-MP377083STK
CSB-EP377083STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP377084STK
CSB-EP377084STK
CSB-BP377084STK
CSB-MP377084STK
CSB-EP377084STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP377085STK
CSB-EP377085STK
CSB-BP377085STK
CSB-MP377085STK
CSB-EP377085STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Putative phosphotransferase Sama_2073 (Sama_2073) CSB-YP377086STK
CSB-EP377086STK
CSB-BP377086STK
CSB-MP377086STK
CSB-EP377086STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Protein TolB (tolB) CSB-YP377087STK
CSB-EP377087STK
CSB-BP377087STK
CSB-MP377087STK
CSB-EP377087STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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