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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Marinobacter aquaeolei tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP378869MNI
CSB-EP378869MNI
CSB-BP378869MNI
CSB-MP378869MNI
CSB-EP378869MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP378870MNI
CSB-EP378870MNI
CSB-BP378870MNI
CSB-MP378870MNI
CSB-EP378870MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Trigger factor (tig) CSB-YP378871MNI
CSB-EP378871MNI
CSB-BP378871MNI
CSB-MP378871MNI
CSB-EP378871MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Cysteine--tRNA ligase (cysS) CSB-YP378872MNI
CSB-EP378872MNI
CSB-BP378872MNI
CSB-MP378872MNI
CSB-EP378872MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Glutamate--tRNA ligase (gltX) CSB-YP378873MNI
CSB-EP378873MNI
CSB-BP378873MNI
CSB-MP378873MNI
CSB-EP378873MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP378874MNI
CSB-EP378874MNI
CSB-BP378874MNI
CSB-MP378874MNI
CSB-EP378874MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Na (+)-translocating NADH-quinone reductase subunit E, partial CSB-YP378875MNI1
CSB-EP378875MNI1
CSB-BP378875MNI1
CSB-MP378875MNI1
CSB-EP378875MNI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Flagellar hook-basal body complex protein FliE (fliE) CSB-YP378876MNI
CSB-EP378876MNI
CSB-BP378876MNI
CSB-MP378876MNI
CSB-EP378876MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei LexA repressor (lexA) CSB-YP378877MNI
CSB-EP378877MNI
CSB-BP378877MNI
CSB-MP378877MNI
CSB-EP378877MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP378878MNI
CSB-EP378878MNI
CSB-BP378878MNI
CSB-MP378878MNI
CSB-EP378878MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP378879MNI
CSB-EP378879MNI
CSB-BP378879MNI
CSB-MP378879MNI
CSB-EP378879MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Probable transcriptional regulatory protein Maqu_2154 (Maqu_2154) CSB-YP378880MNI
CSB-EP378880MNI
CSB-BP378880MNI
CSB-MP378880MNI
CSB-EP378880MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP378881MNI
CSB-EP378881MNI
CSB-BP378881MNI
CSB-MP378881MNI
CSB-EP378881MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP378882MNI
CSB-EP378882MNI
CSB-BP378882MNI
CSB-MP378882MNI
CSB-EP378882MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 50S ribosomal protein L19 (rplS) CSB-YP378883MNI
CSB-EP378883MNI
CSB-BP378883MNI
CSB-MP378883MNI
CSB-EP378883MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei DNA polymerase IV (dinB) CSB-YP378884MNI
CSB-EP378884MNI
CSB-BP378884MNI
CSB-MP378884MNI
CSB-EP378884MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Cell division topological specificity factor (minE) CSB-YP378885MNI
CSB-EP378885MNI
CSB-BP378885MNI
CSB-MP378885MNI
CSB-EP378885MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Peptidyl-tRNA hydrolase (pth) CSB-YP378886MNI
CSB-EP378886MNI
CSB-BP378886MNI
CSB-MP378886MNI
CSB-EP378886MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 50S ribosomal protein L9 (rplI) CSB-YP378887MNI
CSB-EP378887MNI
CSB-BP378887MNI
CSB-MP378887MNI
CSB-EP378887MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Protein translocase subunit SecA (secA), partial CSB-YP378888MNI
CSB-EP378888MNI
CSB-BP378888MNI
CSB-MP378888MNI
CSB-EP378888MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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