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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia mallei Urease accessory protein UreD (ureD) CSB-YP379669BPR
CSB-EP379669BPR
CSB-BP379669BPR
CSB-MP379669BPR
CSB-EP379669BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Undecaprenyl-diphosphatase (uppP), partial CSB-YP379670BPR1
CSB-EP379670BPR1
CSB-BP379670BPR1
CSB-MP379670BPR1
CSB-EP379670BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP379671BPR
CSB-EP379671BPR
CSB-BP379671BPR
CSB-MP379671BPR
CSB-EP379671BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Transcriptional repressor NrdR (nrdR) CSB-YP379672BPR
CSB-EP379672BPR
CSB-BP379672BPR
CSB-MP379672BPR
CSB-EP379672BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 60 kDa chaperonin (groL), partial CSB-YP379673BPR
CSB-EP379673BPR
CSB-BP379673BPR
CSB-MP379673BPR
CSB-EP379673BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Aspartate carbamoyltransferase (pyrB) CSB-YP379674BPR
CSB-EP379674BPR
CSB-BP379674BPR
CSB-MP379674BPR
CSB-EP379674BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0176 protein BMASAVP1_A1042 (BMASAVP1_A1042) CSB-YP379675BPR
CSB-EP379675BPR
CSB-BP379675BPR
CSB-MP379675BPR
CSB-EP379675BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ketol-acid reductoisomerase (ilvC) CSB-YP379676BPR
CSB-EP379676BPR
CSB-BP379676BPR
CSB-MP379676BPR
CSB-EP379676BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP379677BPR1
CSB-EP379677BPR1
CSB-BP379677BPR1
CSB-MP379677BPR1
CSB-EP379677BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0246 protein BMASAVP1_A1161 (BMASAVP1_A1161) CSB-YP379678BPR
CSB-EP379678BPR
CSB-BP379678BPR
CSB-MP379678BPR
CSB-EP379678BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0060 membrane protein BMASAVP1_A1271 (BMASAVP1_A1271), partial CSB-YP379679BPR1
CSB-EP379679BPR1
CSB-BP379679BPR1
CSB-MP379679BPR1
CSB-EP379679BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Putative reductase BMASAVP1_A1410 (BMASAVP1_A1410) CSB-YP379680BPR
CSB-EP379680BPR
CSB-BP379680BPR
CSB-MP379680BPR
CSB-EP379680BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribosome maturation factor RimP (rimP) CSB-YP379681BPR
CSB-EP379681BPR
CSB-BP379681BPR
CSB-MP379681BPR
CSB-EP379681BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Putative arginyl-tRNA--protein transferase (ate) CSB-YP379682BPR
CSB-EP379682BPR
CSB-BP379682BPR
CSB-MP379682BPR
CSB-EP379682BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP379683BPR
CSB-EP379683BPR
CSB-BP379683BPR
CSB-MP379683BPR
CSB-EP379683BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Adenylosuccinate synthetase (purA) CSB-YP379684BPR
CSB-EP379684BPR
CSB-BP379684BPR
CSB-MP379684BPR
CSB-EP379684BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP379685BPR
CSB-EP379685BPR
CSB-BP379685BPR
CSB-MP379685BPR
CSB-EP379685BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 5'-nucleotidase surE (surE) CSB-YP379686BPR
CSB-EP379686BPR
CSB-BP379686BPR
CSB-MP379686BPR
CSB-EP379686BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L9 (rplI) CSB-YP379687BPR
CSB-EP379687BPR
CSB-BP379687BPR
CSB-MP379687BPR
CSB-EP379687BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP379688BPR
CSB-EP379688BPR
CSB-BP379688BPR
CSB-MP379688BPR
CSB-EP379688BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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