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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia mallei 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP379649BPR
CSB-EP379649BPR
CSB-BP379649BPR
CSB-MP379649BPR
CSB-EP379649BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP379650BPR
CSB-EP379650BPR
CSB-BP379650BPR
CSB-MP379650BPR
CSB-EP379650BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0042 nucleotide-binding protein BMASAVP1_A0080 (BMASAVP1_A0080) CSB-YP379651BPR
CSB-EP379651BPR
CSB-BP379651BPR
CSB-MP379651BPR
CSB-EP379651BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phosphopantetheine adenylyltransferase (coaD) CSB-YP379652BPR
CSB-EP379652BPR
CSB-BP379652BPR
CSB-MP379652BPR
CSB-EP379652BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP379653BPR
CSB-EP379653BPR
CSB-BP379653BPR
CSB-MP379653BPR
CSB-EP379653BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Putative iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP379654BPR
CSB-EP379654BPR
CSB-BP379654BPR
CSB-MP379654BPR
CSB-EP379654BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP379655BPR
CSB-EP379655BPR
CSB-BP379655BPR
CSB-MP379655BPR
CSB-EP379655BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Leucine--tRNA ligase (leuS), partial CSB-YP379656BPR
CSB-EP379656BPR
CSB-BP379656BPR
CSB-MP379656BPR
CSB-EP379656BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Dihydrodipicolinate reductase (dapB) CSB-YP379657BPR
CSB-EP379657BPR
CSB-BP379657BPR
CSB-MP379657BPR
CSB-EP379657BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Glutamate 5-kinase (proB) CSB-YP379658BPR
CSB-EP379658BPR
CSB-BP379658BPR
CSB-MP379658BPR
CSB-EP379658BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L21 (rplU) CSB-YP379659BPR
CSB-EP379659BPR
CSB-BP379659BPR
CSB-MP379659BPR
CSB-EP379659BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei D-alanine--D-alanine ligase (ddl) CSB-YP379660BPR
CSB-EP379660BPR
CSB-BP379660BPR
CSB-MP379660BPR
CSB-EP379660BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP379661BPR
CSB-EP379661BPR
CSB-BP379661BPR
CSB-MP379661BPR
CSB-EP379661BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Protein MraZ (mraZ) CSB-YP379662BPR
CSB-EP379662BPR
CSB-BP379662BPR
CSB-MP379662BPR
CSB-EP379662BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chaperone protein DnaK (dnaK), partial CSB-YP379663BPR
CSB-EP379663BPR
CSB-BP379663BPR
CSB-MP379663BPR
CSB-EP379663BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP379664BPR
CSB-EP379664BPR
CSB-BP379664BPR
CSB-MP379664BPR
CSB-EP379664BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP379665BPR
CSB-EP379665BPR
CSB-BP379665BPR
CSB-MP379665BPR
CSB-EP379665BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0234 protein BMASAVP1_A0673 (BMASAVP1_A0673) CSB-YP379666BPR
CSB-EP379666BPR
CSB-BP379666BPR
CSB-MP379666BPR
CSB-EP379666BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Urease accessory protein UreG (ureG) CSB-YP379667BPR
CSB-EP379667BPR
CSB-BP379667BPR
CSB-MP379667BPR
CSB-EP379667BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Urease accessory protein UreE (ureE) CSB-YP379668BPR
CSB-EP379668BPR
CSB-BP379668BPR
CSB-MP379668BPR
CSB-EP379668BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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