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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia mallei ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP379689BPR
CSB-EP379689BPR
CSB-BP379689BPR
CSB-MP379689BPR
CSB-EP379689BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP379690BPR
CSB-EP379690BPR
CSB-BP379690BPR
CSB-MP379690BPR
CSB-EP379690BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ureidoglycolate hydrolase (allA) CSB-YP379691BPR
CSB-EP379691BPR
CSB-BP379691BPR
CSB-MP379691BPR
CSB-EP379691BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribonuclease HII (rnhB) CSB-YP379692BPR
CSB-EP379692BPR
CSB-BP379692BPR
CSB-MP379692BPR
CSB-EP379692BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP379693BPR
CSB-EP379693BPR
CSB-BP379693BPR
CSB-MP379693BPR
CSB-EP379693BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei DNA ligase (ligA), partial CSB-YP379694BPR
CSB-EP379694BPR
CSB-BP379694BPR
CSB-MP379694BPR
CSB-EP379694BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Alanine racemase (alr) CSB-YP379695BPR
CSB-EP379695BPR
CSB-BP379695BPR
CSB-MP379695BPR
CSB-EP379695BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei DNA mismatch repair protein MutS (mutS), partial CSB-YP379696BPR
CSB-EP379696BPR
CSB-BP379696BPR
CSB-MP379696BPR
CSB-EP379696BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Bifunctional protein FolD (folD) CSB-YP379697BPR
CSB-EP379697BPR
CSB-BP379697BPR
CSB-MP379697BPR
CSB-EP379697BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP379698BPR
CSB-EP379698BPR
CSB-BP379698BPR
CSB-MP379698BPR
CSB-EP379698BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Pantothenate synthetase (panC) CSB-YP379699BPR
CSB-EP379699BPR
CSB-BP379699BPR
CSB-MP379699BPR
CSB-EP379699BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Dihydroxy-acid dehydratase (ilvD), partial CSB-YP379700BPR
CSB-EP379700BPR
CSB-BP379700BPR
CSB-MP379700BPR
CSB-EP379700BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP379701BPR
CSB-EP379701BPR
CSB-BP379701BPR
CSB-MP379701BPR
CSB-EP379701BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP379702BPR
CSB-EP379702BPR
CSB-BP379702BPR
CSB-MP379702BPR
CSB-EP379702BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S16 (rpsP) CSB-YP379703BPR
CSB-EP379703BPR
CSB-BP379703BPR
CSB-MP379703BPR
CSB-EP379703BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP379704BPR
CSB-EP379704BPR
CSB-BP379704BPR
CSB-MP379704BPR
CSB-EP379704BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP379705BPR
CSB-EP379705BPR
CSB-BP379705BPR
CSB-MP379705BPR
CSB-EP379705BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Quinolinate synthase A (nadA) CSB-YP379706BPR
CSB-EP379706BPR
CSB-BP379706BPR
CSB-MP379706BPR
CSB-EP379706BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP379707BPR
CSB-EP379707BPR
CSB-BP379707BPR
CSB-MP379707BPR
CSB-EP379707BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP379708BPR
CSB-EP379708BPR
CSB-BP379708BPR
CSB-MP379708BPR
CSB-EP379708BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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