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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia mallei UPF0289 protein BMASAVP1_A0457 (BMASAVP1_A0457) CSB-YP380089BPR
CSB-EP380089BPR
CSB-BP380089BPR
CSB-MP380089BPR
CSB-EP380089BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP380090BPR
CSB-EP380090BPR
CSB-BP380090BPR
CSB-MP380090BPR
CSB-EP380090BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP380091BPR
CSB-EP380091BPR
CSB-BP380091BPR
CSB-MP380091BPR
CSB-EP380091BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP380092BPR
CSB-EP380092BPR
CSB-BP380092BPR
CSB-MP380092BPR
CSB-EP380092BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP380093BPR
CSB-EP380093BPR
CSB-BP380093BPR
CSB-MP380093BPR
CSB-EP380093BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0434 protein BMASAVP1_A0570 (BMASAVP1_A0570) CSB-YP380094BPR
CSB-EP380094BPR
CSB-BP380094BPR
CSB-MP380094BPR
CSB-EP380094BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP380095BPR
CSB-EP380095BPR
CSB-BP380095BPR
CSB-MP380095BPR
CSB-EP380095BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Urease accessory protein UreF (ureF) CSB-YP380096BPR
CSB-EP380096BPR
CSB-BP380096BPR
CSB-MP380096BPR
CSB-EP380096BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Urease subunit gamma (ureA) CSB-YP380097BPR
CSB-EP380097BPR
CSB-BP380097BPR
CSB-MP380097BPR
CSB-EP380097BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Serine--tRNA ligase (serS) CSB-YP380098BPR
CSB-EP380098BPR
CSB-BP380098BPR
CSB-MP380098BPR
CSB-EP380098BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Cell division topological specificity factor (minE) CSB-YP380099BPR
CSB-EP380099BPR
CSB-BP380099BPR
CSB-MP380099BPR
CSB-EP380099BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0226 membrane protein BMASAVP1_A0869 (BMASAVP1_A0869), partial CSB-YP380100BPR
CSB-EP380100BPR
CSB-BP380100BPR
CSB-MP380100BPR
CSB-EP380100BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0301 protein BMASAVP1_A0916 (BMASAVP1_A0916) CSB-YP380101BPR
CSB-EP380101BPR
CSB-BP380101BPR
CSB-MP380101BPR
CSB-EP380101BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP380102BPR
CSB-EP380102BPR
CSB-BP380102BPR
CSB-MP380102BPR
CSB-EP380102BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP380103BPR
CSB-EP380103BPR
CSB-BP380103BPR
CSB-MP380103BPR
CSB-EP380103BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Uracil phosphoribosyltransferase (upp) CSB-YP380104BPR
CSB-EP380104BPR
CSB-BP380104BPR
CSB-MP380104BPR
CSB-EP380104BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP380105BPR
CSB-EP380105BPR
CSB-BP380105BPR
CSB-MP380105BPR
CSB-EP380105BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP380106BPR1
CSB-EP380106BPR1
CSB-BP380106BPR1
CSB-MP380106BPR1
CSB-EP380106BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phosphoglucosamine mutase (glmM) CSB-YP380107BPR
CSB-EP380107BPR
CSB-BP380107BPR
CSB-MP380107BPR
CSB-EP380107BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribosome-binding factor A (rbfA) CSB-YP380108BPR
CSB-EP380108BPR
CSB-BP380108BPR
CSB-MP380108BPR
CSB-EP380108BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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