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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. holarctica Protein-export protein secB 1 (secB1) CSB-YP415173FDV
CSB-EP415173FDV
CSB-BP415173FDV
CSB-MP415173FDV
CSB-EP415173FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Na (+)/H (+) antiporter nhaA, partial CSB-YP415174FDV
CSB-EP415174FDV
CSB-BP415174FDV
CSB-MP415174FDV
CSB-EP415174FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP415175FDV
CSB-EP415175FDV
CSB-BP415175FDV
CSB-MP415175FDV
CSB-EP415175FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Uridylate kinase (pyrH) CSB-YP415176FDV
CSB-EP415176FDV
CSB-BP415176FDV
CSB-MP415176FDV
CSB-EP415176FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L4 (rplD) CSB-YP415177FDV
CSB-EP415177FDV
CSB-BP415177FDV
CSB-MP415177FDV
CSB-EP415177FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L14 (rplN) CSB-YP415178FDV
CSB-EP415178FDV
CSB-BP415178FDV
CSB-MP415178FDV
CSB-EP415178FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L30 (rpmD) CSB-YP415179FDV
CSB-EP415179FDV
CSB-BP415179FDV
CSB-MP415179FDV
CSB-EP415179FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP415180FDV
CSB-EP415180FDV
CSB-BP415180FDV
CSB-MP415180FDV
CSB-EP415180FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Chorismate synthase (aroC) CSB-YP415181FDV
CSB-EP415181FDV
CSB-BP415181FDV
CSB-MP415181FDV
CSB-EP415181FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Methionine--tRNA ligase (metG), partial CSB-YP415182FDV
CSB-EP415182FDV
CSB-BP415182FDV
CSB-MP415182FDV
CSB-EP415182FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Cell division topological specificity factor (minE) CSB-YP415183FDV
CSB-EP415183FDV
CSB-BP415183FDV
CSB-MP415183FDV
CSB-EP415183FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica tRNA pseudouridine synthase B (truB) CSB-YP415184FDV
CSB-EP415184FDV
CSB-BP415184FDV
CSB-MP415184FDV
CSB-EP415184FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP415185FDV
CSB-EP415185FDV
CSB-BP415185FDV
CSB-MP415185FDV
CSB-EP415185FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP415186FDV
CSB-EP415186FDV
CSB-BP415186FDV
CSB-MP415186FDV
CSB-EP415186FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP415187FDV1
CSB-EP415187FDV1
CSB-BP415187FDV1
CSB-MP415187FDV1
CSB-EP415187FDV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP415188FDV
CSB-EP415188FDV
CSB-BP415188FDV
CSB-MP415188FDV
CSB-EP415188FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica tRNA 2-thiocytidine biosynthesis protein TtcA 2 (ttcA2) CSB-YP415189FDV
CSB-EP415189FDV
CSB-BP415189FDV
CSB-MP415189FDV
CSB-EP415189FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Phosphate acyltransferase (plsX) CSB-YP415190FDV
CSB-EP415190FDV
CSB-BP415190FDV
CSB-MP415190FDV
CSB-EP415190FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Protein grpE (grpE) CSB-YP415191FDV
CSB-EP415191FDV
CSB-BP415191FDV
CSB-MP415191FDV
CSB-EP415191FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica UPF0145 protein FTA_1322 (FTA_1322) CSB-YP415192FDV
CSB-EP415192FDV
CSB-BP415192FDV
CSB-MP415192FDV
CSB-EP415192FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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