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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. holarctica Phosphopantetheine adenylyltransferase (coaD) CSB-YP415193FDV
CSB-EP415193FDV
CSB-BP415193FDV
CSB-MP415193FDV
CSB-EP415193FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP415194FDV
CSB-EP415194FDV
CSB-BP415194FDV
CSB-MP415194FDV
CSB-EP415194FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 30S ribosomal protein S15 (rpsO) CSB-YP415195FDV
CSB-EP415195FDV
CSB-BP415195FDV
CSB-MP415195FDV
CSB-EP415195FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Arginine--tRNA ligase (argS), partial CSB-YP415196FDV
CSB-EP415196FDV
CSB-BP415196FDV
CSB-MP415196FDV
CSB-EP415196FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Outer-membrane lipoprotein carrier protein (lolA) CSB-YP415197FDV
CSB-EP415197FDV
CSB-BP415197FDV
CSB-MP415197FDV
CSB-EP415197FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L19 (rplS) CSB-YP415198FDV
CSB-EP415198FDV
CSB-BP415198FDV
CSB-MP415198FDV
CSB-EP415198FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L1 (rplA) CSB-YP415199FDV
CSB-EP415199FDV
CSB-BP415199FDV
CSB-MP415199FDV
CSB-EP415199FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica ATP synthase gamma chain (atpG) CSB-YP415200FDV
CSB-EP415200FDV
CSB-BP415200FDV
CSB-MP415200FDV
CSB-EP415200FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP415201FDV
CSB-EP415201FDV
CSB-BP415201FDV
CSB-MP415201FDV
CSB-EP415201FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Adenylosuccinate synthetase (purA) CSB-YP415202FDV
CSB-EP415202FDV
CSB-BP415202FDV
CSB-MP415202FDV
CSB-EP415202FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Transcription elongation factor GreA (greA) CSB-YP415203RIP
CSB-EP415203RIP
CSB-BP415203RIP
CSB-MP415203RIP
CSB-EP415203RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii S-adenosylmethionine synthase (metK) CSB-YP415204RIP
CSB-EP415204RIP
CSB-BP415204RIP
CSB-MP415204RIP
CSB-EP415204RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Probable transaldolase (tal) CSB-YP415205RIP
CSB-EP415205RIP
CSB-BP415205RIP
CSB-MP415205RIP
CSB-EP415205RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Probable transcriptional regulatory protein Rcas_0718 (Rcas_0718) CSB-YP415206RIP
CSB-EP415206RIP
CSB-BP415206RIP
CSB-MP415206RIP
CSB-EP415206RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Ribosome-binding factor A (rbfA) CSB-YP415207RIP
CSB-EP415207RIP
CSB-BP415207RIP
CSB-MP415207RIP
CSB-EP415207RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Redox-sensing transcriptional repressor rex (rex) CSB-YP415208RIP
CSB-EP415208RIP
CSB-BP415208RIP
CSB-MP415208RIP
CSB-EP415208RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Light-independent protochlorophyllide reductase subunit N (bchN) CSB-YP415209RIP
CSB-EP415209RIP
CSB-BP415209RIP
CSB-MP415209RIP
CSB-EP415209RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Ketol-acid reductoisomerase (ilvC) CSB-YP415210RIP
CSB-EP415210RIP
CSB-BP415210RIP
CSB-MP415210RIP
CSB-EP415210RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Phosphopantetheine adenylyltransferase (coaD) CSB-YP415211RIP
CSB-EP415211RIP
CSB-BP415211RIP
CSB-MP415211RIP
CSB-EP415211RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii UPF0102 protein Rcas_2007 (Rcas_2007) CSB-YP415212RIP
CSB-EP415212RIP
CSB-BP415212RIP
CSB-MP415212RIP
CSB-EP415212RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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