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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Petrotoga mobilis 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP430000PYI
CSB-EP430000PYI
CSB-BP430000PYI
CSB-MP430000PYI
CSB-EP430000PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP430001PYI
CSB-EP430001PYI
CSB-BP430001PYI
CSB-MP430001PYI
CSB-EP430001PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP430002PYI
CSB-EP430002PYI
CSB-BP430002PYI
CSB-MP430002PYI
CSB-EP430002PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Phosphopantetheine adenylyltransferase (coaD) CSB-YP430003PYI
CSB-EP430003PYI
CSB-BP430003PYI
CSB-MP430003PYI
CSB-EP430003PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP430004PYI
CSB-EP430004PYI
CSB-BP430004PYI
CSB-MP430004PYI
CSB-EP430004PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis DNA mismatch repair protein mutL (mutL), partial CSB-YP430005PYI
CSB-EP430005PYI
CSB-BP430005PYI
CSB-MP430005PYI
CSB-EP430005PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP430006PYI
CSB-EP430006PYI
CSB-BP430006PYI
CSB-MP430006PYI
CSB-EP430006PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP430007PYI
CSB-EP430007PYI
CSB-BP430007PYI
CSB-MP430007PYI
CSB-EP430007PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP430008DHV
CSB-EP430008DHV
CSB-BP430008DHV
CSB-MP430008DHV
CSB-EP430008DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans UPF0761 membrane protein Daci_4966 (Daci_4966), partial CSB-YP430009DHV1
CSB-EP430009DHV1
CSB-BP430009DHV1
CSB-MP430009DHV1
CSB-EP430009DHV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP430010DHV
CSB-EP430010DHV
CSB-BP430010DHV
CSB-MP430010DHV
CSB-EP430010DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 30S ribosomal protein S18 (rpsR) CSB-YP430011DHV
CSB-EP430011DHV
CSB-BP430011DHV
CSB-MP430011DHV
CSB-EP430011DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Tryptophan synthase alpha chain (trpA) CSB-YP430012DHV
CSB-EP430012DHV
CSB-BP430012DHV
CSB-MP430012DHV
CSB-EP430012DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP430013DHV
CSB-EP430013DHV
CSB-BP430013DHV
CSB-MP430013DHV
CSB-EP430013DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 30S ribosomal protein S16 (rpsP) CSB-YP430014DHV
CSB-EP430014DHV
CSB-BP430014DHV
CSB-MP430014DHV
CSB-EP430014DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP430015DHV
CSB-EP430015DHV
CSB-BP430015DHV
CSB-MP430015DHV
CSB-EP430015DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Glutamate 5-kinase (proB) CSB-YP430016DHV
CSB-EP430016DHV
CSB-BP430016DHV
CSB-MP430016DHV
CSB-EP430016DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 30S ribosomal protein S7 (rpsG) CSB-YP430017DHV
CSB-EP430017DHV
CSB-BP430017DHV
CSB-MP430017DHV
CSB-EP430017DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L29 (rpmC) CSB-YP430018DHV
CSB-EP430018DHV
CSB-BP430018DHV
CSB-MP430018DHV
CSB-EP430018DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Alanine racemase (alr) CSB-YP430019DHV
CSB-EP430019DHV
CSB-BP430019DHV
CSB-MP430019DHV
CSB-EP430019DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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