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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia multivorans NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP432562BPS
CSB-EP432562BPS
CSB-BP432562BPS
CSB-MP432562BPS
CSB-EP432562BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Protein grpE (grpE) CSB-YP432563BPS
CSB-EP432563BPS
CSB-BP432563BPS
CSB-MP432563BPS
CSB-EP432563BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP432564BPS1
CSB-EP432564BPS1
CSB-BP432564BPS1
CSB-MP432564BPS1
CSB-EP432564BPS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP432565BPS
CSB-EP432565BPS
CSB-BP432565BPS
CSB-MP432565BPS
CSB-EP432565BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Co-chaperone protein hscB homolog (hscB) CSB-YP432566BPS
CSB-EP432566BPS
CSB-BP432566BPS
CSB-MP432566BPS
CSB-EP432566BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Recombination protein RecR (recR) CSB-YP432567BPS
CSB-EP432567BPS
CSB-BP432567BPS
CSB-MP432567BPS
CSB-EP432567BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP432568BPS
CSB-EP432568BPS
CSB-BP432568BPS
CSB-MP432568BPS
CSB-EP432568BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Dihydrodipicolinate reductase (dapB) CSB-YP432569BPS
CSB-EP432569BPS
CSB-BP432569BPS
CSB-MP432569BPS
CSB-EP432569BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Cysteine--tRNA ligase (cysS) CSB-YP432570BPS
CSB-EP432570BPS
CSB-BP432570BPS
CSB-MP432570BPS
CSB-EP432570BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Ribosomal protein L11 methyltransferase (prmA) CSB-YP432571BPS
CSB-EP432571BPS
CSB-BP432571BPS
CSB-MP432571BPS
CSB-EP432571BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Protein translocase subunit SecA (secA), partial CSB-YP432572BPS
CSB-EP432572BPS
CSB-BP432572BPS
CSB-MP432572BPS
CSB-EP432572BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP432573BPS
CSB-EP432573BPS
CSB-BP432573BPS
CSB-MP432573BPS
CSB-EP432573BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP432574BPS
CSB-EP432574BPS
CSB-BP432574BPS
CSB-MP432574BPS
CSB-EP432574BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans ATP synthase gamma chain (atpG) CSB-YP432575BPS
CSB-EP432575BPS
CSB-BP432575BPS
CSB-MP432575BPS
CSB-EP432575BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Potassium-transporting ATPase C chain (kdpC), partial CSB-YP432576BPS
CSB-EP432576BPS
CSB-BP432576BPS
CSB-MP432576BPS
CSB-EP432576BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP432577BPS
CSB-EP432577BPS
CSB-BP432577BPS
CSB-MP432577BPS
CSB-EP432577BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Dihydroorotate dehydrogenase (quinone) CSB-YP432578BPS
CSB-EP432578BPS
CSB-BP432578BPS
CSB-MP432578BPS
CSB-EP432578BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP432579BPS
CSB-EP432579BPS
CSB-BP432579BPS
CSB-MP432579BPS
CSB-EP432579BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP432580BPS
CSB-EP432580BPS
CSB-BP432580BPS
CSB-MP432580BPS
CSB-EP432580BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0391 membrane protein Bmul_5473/BMULJ_06024 (Bmul_5473, BMULJ_06024) CSB-YP432581BPS
CSB-EP432581BPS
CSB-BP432581BPS
CSB-MP432581BPS
CSB-EP432581BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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