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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Petrotoga mobilis S-adenosylmethionine synthase (metK) CSB-YP432642PYI
CSB-EP432642PYI
CSB-BP432642PYI
CSB-MP432642PYI
CSB-EP432642PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Acetylglutamate kinase (argB) CSB-YP432643PYI
CSB-EP432643PYI
CSB-BP432643PYI
CSB-MP432643PYI
CSB-EP432643PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Bifunctional protein FolD (folD) CSB-YP432644PYI
CSB-EP432644PYI
CSB-BP432644PYI
CSB-MP432644PYI
CSB-EP432644PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Glutamate--tRNA ligase 1 (gltX1) CSB-YP432645PYI
CSB-EP432645PYI
CSB-BP432645PYI
CSB-MP432645PYI
CSB-EP432645PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP432646PYI
CSB-EP432646PYI
CSB-BP432646PYI
CSB-MP432646PYI
CSB-EP432646PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP432647PYI
CSB-EP432647PYI
CSB-BP432647PYI
CSB-MP432647PYI
CSB-EP432647PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Phosphoglucosamine mutase (glmM) CSB-YP432648DHV
CSB-EP432648DHV
CSB-BP432648DHV
CSB-MP432648DHV
CSB-EP432648DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP432649DHV
CSB-EP432649DHV
CSB-BP432649DHV
CSB-MP432649DHV
CSB-EP432649DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP432650DHV
CSB-EP432650DHV
CSB-BP432650DHV
CSB-MP432650DHV
CSB-EP432650DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Chaperone protein DnaK (dnaK), partial CSB-YP432651DHV
CSB-EP432651DHV
CSB-BP432651DHV
CSB-MP432651DHV
CSB-EP432651DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP432652DHV
CSB-EP432652DHV
CSB-BP432652DHV
CSB-MP432652DHV
CSB-EP432652DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans UPF0246 protein Daci_5283 (Daci_5283) CSB-YP432653DHV
CSB-EP432653DHV
CSB-BP432653DHV
CSB-MP432653DHV
CSB-EP432653DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Heat-inducible transcription repressor HrcA (hrcA) CSB-YP432654DHV
CSB-EP432654DHV
CSB-BP432654DHV
CSB-MP432654DHV
CSB-EP432654DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Aspartate--tRNA ligase (aspS), partial CSB-YP432655DHV
CSB-EP432655DHV
CSB-BP432655DHV
CSB-MP432655DHV
CSB-EP432655DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L21 (rplU) CSB-YP432656DHV
CSB-EP432656DHV
CSB-BP432656DHV
CSB-MP432656DHV
CSB-EP432656DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L4 (rplD) CSB-YP432657DHV
CSB-EP432657DHV
CSB-BP432657DHV
CSB-MP432657DHV
CSB-EP432657DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans ATP synthase subunit c (atpE), partial CSB-YP432658DHV1
CSB-EP432658DHV1
CSB-BP432658DHV1
CSB-MP432658DHV1
CSB-EP432658DHV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP432659DHV
CSB-EP432659DHV
CSB-BP432659DHV
CSB-MP432659DHV
CSB-EP432659DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP432660DHV
CSB-EP432660DHV
CSB-BP432660DHV
CSB-MP432660DHV
CSB-EP432660DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L30 (rpmD) CSB-YP432661DHV
CSB-EP432661DHV
CSB-BP432661DHV
CSB-MP432661DHV
CSB-EP432661DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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