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Bovine Immunoglobulin M,IgM ELISA Kit

  • 中文名稱(chēng):
    牛免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E12017B
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    牛免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒(CSB-E12017B)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IgM含量。IgM是人體內(nèi)最早出現(xiàn)的免疫球蛋白,具有強(qiáng)大的殺菌和免疫調(diào)節(jié)功能。它主要通過(guò)B細(xì)胞受體(BCR)在早期免疫應(yīng)答中發(fā)揮作用,并以五聚體或六聚體的形式存在。研究顯示,IgM的組裝和轉(zhuǎn)運(yùn)受J鏈調(diào)控,并與黏膜轉(zhuǎn)運(yùn)受體pIgR相互作用,參與黏膜免疫。近期研究揭示了IgM被特異性受體FcμR識(shí)別的分子機(jī)制,為深入理解其生物學(xué)功能提供了重要基礎(chǔ)。試劑盒檢測(cè)范圍為0.39 μg/mL-25 μg/mL,靈敏度為0.2 μg/mL。適合用于科研領(lǐng)域評(píng)估牛類(lèi)動(dòng)物免疫機(jī)能、疫苗研發(fā)中抗體應(yīng)答動(dòng)態(tài)監(jiān)測(cè),或感染性疾病模型中特異性IgM水平分析,為免疫學(xué)研究提供可靠的定量工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    IgM
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類(lèi)型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍:
    0.39 μg/mL-25 μg/mL
  • 靈敏度:
    0.2 μg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 86-99  
    1:200 Average % 97  
    Range % 92-103  
    1:400 Average % 85  
    Range % 81-87  
    1:800 Average % 89  
    Range % 83-95  
  • 回收率:
    The recovery of bovine IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
    EDTA plasma (n=4) 97 92-102  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    25 0.059 0.056 0.058    
    12.5 0.091 0.099 0.095    
    6.25 0.151 0.152 0.152    
    3.12 0.312 0.292 0.302    
    1.56 0.608 0.660 0.634    
    0.78 0.824 0.801 0.813    
    0.39 1.150 1.192 1.171    
    0 1.552 1.508 1.530    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    IgM是一種免疫球蛋白,被認(rèn)為是先天免疫系統(tǒng)的一個(gè)關(guān)鍵組成部分。最近的研究表明,IgM在多種疾病的預(yù)防和治療中具有重要作用。例如,在新冠疫情期間,研究人員使用IgM抗體來(lái)診斷和治療感染。此外,IgM還被用于治療其他疾病,如類(lèi)風(fēng)濕關(guān)節(jié)炎和多發(fā)性硬化癥。