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  4. Bovine Trypsin ELISA Kit

Bovine Trypsin ELISA Kit

  • 中文名稱：
    牛胰蛋白酶酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL018811BO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛胰蛋白酶酶聯(lián)免疫試劑盒（CSB-EL018811BO）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Trypsin含量。Trypsin即胰蛋白酶，是一種在消化過(guò)程中起關(guān)鍵作用的絲氨酸蛋白酶，主要由胰腺分泌。其研究機(jī)制主要聚焦在它對(duì)肽鍵的水解作用，參與蛋白質(zhì)的消化。此外在細(xì)胞培養(yǎng)、疾病診斷和治療方面也有研究應(yīng)用。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，主要應(yīng)用于畜牧科研中反芻動(dòng)物消化生理研究、胰酶代謝調(diào)控機(jī)制探索，或生物制藥領(lǐng)域酶制劑活性檢測(cè)等場(chǎng)景，為蛋白質(zhì)代謝相關(guān)的基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫：
    Trypsin
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of bovine trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:100

    Average %

    89

    Range %

    82-96

    1:200

    Average %

    98

    Range %

    91-105

    1:400

    Average %

    87

    Range %

    82-94

    1:800

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of bovine trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    89

    85-95

    EDTA plasma (n=4)

    93

    89-98

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    4000

    0.180

    0.174

    0.177

    2000

    0.275

    0.267

    0.271

    1000

    0.429

    0.420

    0.424

    500

    0.613

    0.629

    0.621

    250

    0.985

    1.021

    1.003

    125

    1.581

    1.653

    1.617

    62.5

    2.093

    2.184

    2.139

    0

    2.652

    2.725

    2.688

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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