Mouse trypsin ELISA Kit
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中文名稱:小鼠胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E09900m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒(CSB-E09900m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的trypsin含量。胰蛋白酶是一種絲氨酸蛋白酶,主要在消化系統(tǒng)中將蛋白質(zhì)分解為肽和氨基酸。它通過(guò)特異性切割賴氨酸和精氨酸殘基的肽鍵來(lái)發(fā)揮作用,是蛋白質(zhì)消化和蛋白質(zhì)組學(xué)研究中不可或缺的工具酶。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL,適用于科研領(lǐng)域中對(duì)胰蛋白酶表達(dá)水平的定量分析,例如研究胰腺疾病模型中的酶活性變化、評(píng)估細(xì)胞培養(yǎng)體系中胰蛋白酶的釋放規(guī)律,或分析組織樣本在特定生理病理?xiàng)l件下的蛋白酶動(dòng)態(tài)。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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縮寫:trypsin
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍:1.56 ng/mL-100 ng/mL
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靈敏度:0.39 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 89-95 1:2 Average % 103 Range % 99-107 1:4 Average % 92 Range % 87-96 1:8 Average % 98 Range % 94-101 -
回收率:
The recovery of mouse trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-96 EDTA plasma (n=4) 95 89-98 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 100 2.219 2.112 2.166 2.010 50 1.624 1.576 1.600 1.444 25 1.001 0.991 0.996 0.840 12.5 0.679 0.666 0.673 0.517 6.25 0.377 0.356 0.367 0.211 3.12 0.304 0.313 0.309 0.153 1.56 0.260 0.273 0.267 0.111 0 0.155 0.157 0.156 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Antitumor Activity of Tenacissoside H on Esophageal Cancer through Arresting Cell Cycle and Regulating PI3K/Akt-NF-κB Transduction Cascade. Jia YS.et al,Evid Based Complement Alternat Med.,2015
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