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  4. Pig Trypsin ELISA Kit

Pig Trypsin ELISA Kit

  • 中文名稱：
    豬胰蛋白酶(Trypsin)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E15767p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬胰蛋白酶(Trypsin)酶聯(lián)免疫試劑盒（CSB-E15767p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Trypsin含量。Trypsin即胰蛋白酶，是一種絲氨酸蛋白酶，廣泛存在于脊椎動物消化系統(tǒng)中。其能特異性切割精氨酸和賴氨酸殘基羧基端肽鍵。在研究機制上，常作為工具酶用于蛋白質(zhì)分析，通過切割蛋白質(zhì)來確定其結(jié)構(gòu)和功能 。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，可用于動物模型研究、胰腺外分泌功能評估、飼料營養(yǎng)代謝分析或酶活性相關(guān)機制探索等科研場景，為消化生理學(xué)、病理學(xué)及生物醫(yī)藥開發(fā)提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    Trypsin
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %89
    Range %83-97
    1:10Average %85
    Range %80-90
    1:20Average %90
    Range %84-99
    1:40Average %93
    Range %88-100
  • 回收率：
    The recovery of pig trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9893-103
    EDTA plasma (n=4)9994-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.192 2.131 2.162 2.029
    1001.769 1.834 1.802 1.669
    501.289 1.319 1.304 1.171
    250.824 0.867 0.846 0.713
    12.50.543 0.577 0.560 0.427
    6.250.372 0.337 0.355 0.222
    3.120.288 0.259 0.274 0.141
    00.137 0.129 0.133 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價