Canine Prostaglandin E2,PG-E2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬前列腺素E2(PGE2)酶聯(lián)免疫試劑盒（CSB-E13493c）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PG-E2含量。PG-E2即前列腺素E2，在人體生理與病理過程中廣泛參與。它參與炎癥反應(yīng)、免疫調(diào)節(jié)等，還與腫瘤微環(huán)境形成相關(guān)。研究機(jī)制上，主要通過與細(xì)胞表面受體結(jié)合，激活信號通路，影響細(xì)胞增殖、凋亡等過程，是疾病治療研究的潛在靶點。試劑盒檢測范圍為10 pg/mL-2000 pg/mL，該產(chǎn)品特別適用于研究犬類炎癥性疾?。ㄈ珀P(guān)節(jié)炎、胃腸炎）、免疫應(yīng)答機(jī)制、藥物抗炎效果評估，以及生殖系統(tǒng)或腫瘤相關(guān)研究中的PGE2動態(tài)監(jiān)測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PG-E2
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

10 pg/mL-2000 pg/mL
靈敏度：

5 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-104
1:2	Average %	95
	Range %	89-105
1:4	Average %	93
	Range %	87-100
1:8	Average %	95
	Range %	90-101

回收率：

The recovery of canine PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-96
EDTA plasma (n=4)	96	89-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.