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Rat Prostaglandin E2,PG-E2 ELISA Kit

  • 中文名稱:
    大鼠前列腺素E2(PGE2)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E07967r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠前列腺素E2(PGE2)酶聯(lián)免疫試劑盒(CSB-E07967r)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的PG-E2含量。PG-E2即前列腺素E2,它在體內(nèi)廣泛存在,參與多種生理和病理過程。在炎癥、免疫調(diào)節(jié)、腫瘤發(fā)生發(fā)展等方面扮演重要角色。研究機(jī)制主要圍繞其與受體結(jié)合,激活多條信號(hào)通路,影響細(xì)胞增殖、分化和炎癥因子釋放等過程。試劑盒檢測(cè)范圍為0.5 pg/mL-100 pg/mL,適用于體外實(shí)驗(yàn)研究,例如探究炎癥模型中PGE2的動(dòng)態(tài)變化、評(píng)估藥物或化合物對(duì)前列腺素代謝通路的影響,以及組織微環(huán)境中脂質(zhì)介質(zhì)的定量分析等科研場(chǎng)景,為炎癥機(jī)制研究、藥效學(xué)評(píng)價(jià)及生物標(biāo)志物探索提供高效檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    PG-E2
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍:
    0.5 pg/mL-100 pg/mL
  • 靈敏度:
    0.25 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 91-99  
    1:2 Average % 92  
    Range % 87-96  
    1:4 Average % 93  
    Range % 89-98  
    1:8 Average % 105  
    Range % 99-109  
  • 回收率:
    The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-99  
    EDTA plasma (n=4) 101 96-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    100 0.228 0.224 0.226    
    25 0.365 0.348 0.357    
    8 0.560 0.539 0.550    
    2 0.813 0.787 0.800    
    0.5 1.258 1.234 1.246    
    0 2.031 2.176 2.104    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    PGE2是一種前列腺素,與炎癥和癌癥等多種疾病有關(guān)。近期的研究表明,PGE2可以在體內(nèi)誘導(dǎo)肝癌干細(xì)胞分化為肝癌細(xì)胞,從而促進(jìn)肝癌的發(fā)生和發(fā)展。此外,研究人員還發(fā)現(xiàn),通過靶向PGE2的合成酶和受體可以減少腫瘤的生長(zhǎng)和轉(zhuǎn)移,從而為腫瘤治療提供新的策略。