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  4. Human Prostaglandin E2,PG-E2 ELISA Kit

Human Prostaglandin E2,PG-E2 ELISA Kit

  • 中文名稱：
    人前列腺素E2(PGE2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07965h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人前列腺素E2(PGE2)酶聯(lián)免疫試劑盒（CSB-E07965h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PG-E2含量。PG-E2（前列腺素 E2）是一種生物活性脂質(zhì)介質(zhì)。它在炎癥反應(yīng)、免疫調(diào)節(jié)、組織修復(fù)等多種生理和病理過(guò)程中發(fā)揮重要作用。其研究機(jī)制主要聚焦于與特定受體結(jié)合，激活細(xì)胞內(nèi)信號(hào)通路，從而影響細(xì)胞的增殖、分化和功能，對(duì)相關(guān)疾病治療研究有重要意義。試劑盒檢測(cè)范圍為1.5 pg/mL-300 pg/mL，本試劑盒可為體外研究炎癥介導(dǎo)機(jī)制、免疫調(diào)節(jié)通路、藥物干預(yù)效果評(píng)估等提供可靠數(shù)據(jù)支持，特別適用于實(shí)驗(yàn)室條件下探索生物樣本中PGE2的動(dòng)態(tài)變化規(guī)律，滿足細(xì)胞實(shí)驗(yàn)、動(dòng)物模型或離體組織分析等基礎(chǔ)科研需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫：
    PG-E2
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.5 pg/mL-300 pg/mL
  • 靈敏度：
    0.75 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-99  
    1:2 Average % 92  
    Range % 86-96  
    1:4 Average % 97  
    Range % 92-103  
    1:8 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of human PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 90 83-94  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    300 0.193 0.201 0.197    
    75 0.349 0.327 0.338    
    24 0.523 0.531 0.527    
    6 0.903 0.962 0.933    
    1.5 1.316 1.393 1.355    
    0 2.271 2.212 2.242    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問(wèn)答

■ 常見問(wèn)題解答
Q:

Can this kit test cell supernatnat?

A:
Thanks for your inquiry. Usually we suggest you test the sample types mentioned in the kit manual. If you want to test cell supernatnat samples, you can do a pretest first.