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  4. Fish immunoglobulin M (IgM) ELISA Kit

Fish immunoglobulin M (IgM) ELISA Kit

  • 中文名稱：
    魚免疫球蛋白IgM酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12045Fh
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    魚免疫球蛋白IgM酶聯(lián)免疫試劑盒（CSB-E12045Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IgM含量。IgM是人體最早產生的免疫球蛋白，在早期免疫防御中發(fā)揮關鍵作用。它可通過多種機制清除病原體，如激活補體系統(tǒng)介導溶菌等。在研究方面，其作為靶點被用于多種疾病如感染性疾病、自身免疫病的治療藥物研發(fā)等。試劑盒檢測范圍為1.25 μg/mL-50 μg/mL，適用于水產養(yǎng)殖研究、環(huán)境毒理學評估及魚類免疫機制探索，例如監(jiān)測疫苗免疫后抗體應答、評估水質污染物對免疫功能的影響，或解析疾病模型中的免疫動態(tài)變化。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 縮寫：
    IgM
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.25 μg/mL-50 μg/mL
  • 靈敏度：
    1.25 μg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 86-101  
    1:2 Average % 97  
    Range % 90-104  
    1:4 Average % 95  
    Range % 84-105  
    1:8 Average % 95  
    Range % 91-99  
  • 回收率：
    The recovery of fish IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-105  
    EDTA plasma (n=4) 95 91-102  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    50 0.122 0.124 0.123    
    25 0.213 0.199 0.206    
    11.5 0.401 0.427 0.414    
    3.75 0.745 0.761 0.753    
    1.25 1.375 1.402 1.389    
    0 2.541 2.532 2.537    
  • 本試劑盒所含材料：
      • A 96-well Assay plate --The 96-well plate has been pre-coated with goat-anti-rabbit antibody.
      • Standard (6 x 0.5 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated IgM (1 x 6 ml) --Bind to the IgM antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
      • IgM Antibody (1 x 6 ml) --Interact with the IgM.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

相關問答

■ 常見問題解答
Q:

CUSABIO fish immnoglobulin M (IgM) ELISA kit Does this kit can be used for separate experiments?around 30-40 wells every time?

A:
Thanks for your inquiry.
#CSB-E12045Fh,Fish immunoglobulin M (IgM) ELISA Kit
YES,it can.The plate is Breakapart and can be used for seperate experiments.You can put the break part in the
Aluminum foil bag for next use.The manual is provided to you by e-mail.Additionally,we received 21 published papers until now.You can refer to it. Pls let me know if you have any further questions. Thank you.
Q:

Regarding your ELISA kit CSB-E12045Fh, could you please advise on the following:
1. Capture Antibody: host animal species, clonality
2. Detection Antibody: host animal species, clonality
3. Standard
Also, what sample types have been tested with this kit, and what were the reference values?

A:
Thanks for your inquiry.
CSB-E12045Fh
The target is a special protein and there is no uniprot.
This kit is based on competitive method. The plate is coated with goat-anti-rabbit antibody. The specific antibody is rabbit polyclonal antibody. The standard is purified crude extract of natural samples.
We tested undiluted serum and plasma before and the test value is 1.25--3.75μg/ml.
Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 最新研究進展：
    IgM是體液免疫系統(tǒng)中最早產生的抗體，通常作為感染初期的主要抗體。最新研究表明，IgM除了參與傳統(tǒng)的抗體介導的免疫反應外，還具有其他生物學功能，如參與調節(jié)免疫細胞的增殖和凋亡等。此外，研究人員正在探索利用IgM抗體治療感染和自身免疫性疾病等疾病的潛在機制和應用前景。