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Fish lysozyme (renal amyloidosis) (LZM) ELISA kit

  • 中文名稱(chēng):
    魚(yú)溶菌酶(腎淀粉樣變)(LZM)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E17296Fh
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    魚(yú)溶菌酶(腎淀粉樣變)(LZM)酶聯(lián)免疫試劑盒(CSB-E17296Fh)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的LZM含量。LZM即溶菌酶,是一種能水解致病菌中黏多糖的堿性酶。廣泛存在于動(dòng)植物及微生物中,具有抗菌、抗病毒等特性。研究機(jī)制主要是通過(guò)破壞細(xì)菌細(xì)胞壁的肽聚糖,使細(xì)菌溶解死亡,在醫(yī)藥、食品等領(lǐng)域有潛在應(yīng)用價(jià)值。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL,該產(chǎn)品主要應(yīng)用于水產(chǎn)養(yǎng)殖中的魚(yú)類(lèi)健康監(jiān)測(cè)、免疫相關(guān)疾病機(jī)制研究以及水質(zhì)污染對(duì)魚(yú)類(lèi)生理影響的評(píng)估等領(lǐng)域,為實(shí)驗(yàn)室開(kāi)展魚(yú)類(lèi)病理學(xué)、毒理學(xué)及免疫學(xué)研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    LZM
  • 種屬:
    Fish
  • 樣本類(lèi)型:
    serum, plasma
  • 檢測(cè)范圍:
    3.12 ng/mL-200 ng/mL
  • 靈敏度:
    1.56 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 93  
    Range % 85-100  
    1:40 Average % 91  
    Range % 86-99  
    1:80 Average % 95  
    Range % 90-103  
    1:160 Average % 95  
    Range % 90-98  
  • 回收率:
    The recovery of fish LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-94  
    EDTA plasma (n=4) 93 85-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    200 0.088 0.090 0.089    
    100 0.206 0.194 0.200    
    50 0.307 0.300 0.304    
    25 0.418 0.423 0.421    
    12.5 0.627 0.605 0.616    
    6.25 1.045 1.031 1.038    
    3.12 1.648 1.524 1.586    
    0 2.685 2.657 2.671    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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