Mouse Lysozyme,LZM ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠溶菌酶(LZM)酶聯(lián)免疫試劑盒（CSB-E11329m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LZM含量。LZM（溶菌酶）它是一種天然抗菌物質(zhì)，廣泛存在于多種生物體內(nèi)。研究機(jī)制上，其能特異性地作用于細(xì)菌細(xì)胞壁肽聚糖，破壞細(xì)胞壁結(jié)構(gòu)，導(dǎo)致細(xì)菌溶解死亡，在醫(yī)藥、食品等領(lǐng)域有潛在應(yīng)用價(jià)值。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，該產(chǎn)品可廣泛應(yīng)用于小鼠感染性疾病模型、免疫系統(tǒng)功能評(píng)估、炎癥機(jī)制研究以及藥物干預(yù)實(shí)驗(yàn)中溶菌酶動(dòng)態(tài)變化的檢測(cè)，為科研人員探究先天免疫應(yīng)答機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

LZM
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	89-98
1:200	Average %	97
	Range %	94-101
1:400	Average %	98
	Range %	93-103
1:800	Average %	93
	Range %	87-100

回收率：

The recovery of mouse LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-100
EDTA plasma (n=4)	91	85-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.