Goat Immunoglobulin A(IgA) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

山羊免疫球蛋白A(IgA)酶聯(lián)免疫試劑盒（CSB-E13135G）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgA含量。IgA即免疫球蛋白A，是人體黏膜免疫的重要組成部分，在抵御病原體入侵等方面發(fā)揮關(guān)鍵作用。相關(guān)研究機(jī)制主要圍繞其結(jié)構(gòu)與功能的關(guān)系，探索如何增強(qiáng)IgA的免疫活性、研發(fā)基于IgA靶點(diǎn)的新型治療方法，以應(yīng)對(duì)感染性疾病、自身免疫病等。試劑盒檢測范圍為29 ng/mL-7500 ng/mL，該產(chǎn)品適用于動(dòng)物免疫學(xué)研究，如疫苗接種效果評(píng)價(jià)、病原感染后免疫應(yīng)答監(jiān)測、營養(yǎng)干預(yù)對(duì)黏膜免疫的影響分析等方向，也可用于比較醫(yī)學(xué)中反芻動(dòng)物疾病模型的免疫機(jī)制探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IgA
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma
檢測范圍：

29 ng/mL-7500 ng/mL
靈敏度：

29 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat immunoglobulin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	94
	Range %	90-103
1:10000	Average %	96
	Range %	92-100
1:20000	Average %	90
	Range %	85-97
1:40000	Average %	92
	Range %	85-103

回收率：

The recovery of goat immunoglobulin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	95	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.