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Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit

  • 中文名稱(chēng):
    人Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08087h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒(CSB-E08087h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的Col Ⅱ含量。Col Ⅱ,即II型膠原蛋白,是構(gòu)成透明軟骨的基礎(chǔ),由膠原蛋白II型α1鏈的同源三聚體組成。它在軟骨中占比高達(dá)50%,負(fù)責(zé)提供組織的拉伸強(qiáng)度和蛋白聚糖的捕獲。在研究中,Col Ⅱ的合成和分泌在體外培養(yǎng)的軟骨細(xì)胞中持續(xù)存在,但其合成在細(xì)胞快速增殖時(shí)會(huì)被關(guān)閉,這為組織工程軟骨提供了新的策略。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL,適用于體外科研實(shí)驗(yàn)中定量分析Col Ⅱ的表達(dá)動(dòng)態(tài),例如研究軟骨組織工程材料的生物相容性、關(guān)節(jié)炎模型中基質(zhì)代謝變化、軟骨細(xì)胞分化調(diào)控機(jī)制等。為軟骨相關(guān)疾病機(jī)制探索、藥物干預(yù)效果評(píng)估等基礎(chǔ)研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
  • 縮寫(xiě):
    Col Ⅱ
  • 種屬:
    Homo sapiens (Human)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 86  
    Range % 80-90  
    1:200 Average % 93  
    Range % 88-98  
    1:400 Average % 94  
    Range % 90-100  
    1:800 Average % 96  
    Range % 90-102  
  • 回收率:
    The recovery of human Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-98  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.478 2.387 2.433 2.341  
    1000 1.811 1.752 1.782 1.690  
    500 1.237 1.142 1.190 1.098  
    250 0.863 0.813 0.838 0.746  
    125 0.398 0.374 0.386 0.294  
    62.5 0.211 0.198 0.205 0.113  
    31.25 0.145 0.141 0.143 0.051  
    0 0.093 0.090 0.092    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

人膠原蛋白型Ⅱ,ColⅡELISA試劑盒(Code:CSB-E08087h)是否只檢測(cè)天然(未變性)II型膠原蛋白,還是也檢測(cè)變性II型膠原蛋白?

A:
CSB-E08087h試劑盒檢測(cè)天然(未變性)人Ⅱ型膠原蛋白沒(méi)有問(wèn)題。 對(duì)于變性狀態(tài),理論上會(huì)對(duì)蛋白質(zhì)結(jié)構(gòu)抗體識(shí)別區(qū)域有影響。