Rat Collagen Type Ⅱ,Col Ⅱ ELISA Kit

產(chǎn)品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒（CSB-E08092r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的Col Ⅱ含量。Col Ⅱ即Ⅱ型膠原，是軟骨基質(zhì)的主要成分。其與關(guān)節(jié)炎等疾病密切相關(guān)，自身抗體識別可觸發(fā)免疫反應(yīng)。研究機制主要圍繞其在軟骨穩(wěn)態(tài)維持、免疫調(diào)節(jié)中的作用，探索其在疾病診斷、治療靶點方面的價值。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品可廣泛應(yīng)用于軟骨發(fā)育、退行性關(guān)節(jié)疾病機制研究、藥物或生物材料對軟骨代謝影響的體外評估等科研領(lǐng)域，為探索Col Ⅱ在骨骼系統(tǒng)相關(guān)疾病中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
縮寫：

Col Ⅱ
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	83-92
1:200	Average %	94
	Range %	90-99
1:400	Average %	92
	Range %	88-96
1:800	Average %	102
	Range %	98-105

回收率：

The recovery of rat Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	93-96
EDTA plasma (n=4)	98	89-104

標(biāo)準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.