Mouse Collagen Type Ⅱ,Col Ⅱ ELISA Kit

產(chǎn)品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒（CSB-E08089m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅱ含量。小鼠Ⅱ型膠原（Col Ⅱ）是軟骨的主要成分，具有維持軟骨組織完整性和促進軟骨細胞生長的功能。其研究背景涉及基因工程技術(shù)，通過畢赤酵母表達重組Col Ⅱ，用于凝血和組織再生。研究機制包括Col Ⅱ在促進凝血、成纖維細胞分化中的應(yīng)用，以及其在治療骨關(guān)節(jié)炎等疾病中的作用。試劑盒檢測范圍為1.87 ng/mL-120 ng/mL，適用于科研領(lǐng)域中小鼠關(guān)節(jié)炎模型評估、軟骨發(fā)育機制研究或退行性關(guān)節(jié)疾病中Col Ⅱ代謝動態(tài)分析，為體外實驗提供高特異性的定量檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
縮寫：

Col Ⅱ
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.87 ng/mL-120 ng/mL
靈敏度：

0.46 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	101
	Range %	94-105
1:4	Average %	90
	Range %	85-94
1:8	Average %	97
	Range %	91-100

回收率：

The recovery of mouse Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-93
EDTA plasma (n=4)	104	95-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.